Publication

Fibronectin aggregation in multiple sclerosis lesions impairs remyelination

Stoffels, J. M. J., de Jonge, J. C., Stancic, M., Nomden, A., van Strien, M. E., Ma, D., Siskova, Z., Maier, O., Ffrench-Constant, C., Franklin, R. J. M., Hoekstra, D., Zhao, C. & Baron, W., Jan-2013, In : Brain. 136, p. 116-131 16 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Josephine M. J. Stoffels
  • Jenny C. de Jonge
  • Mirjana Stancic
  • Anita Nomden
  • Miriam E. van Strien
  • Dan Ma
  • Zuzana Siskova
  • Olaf Maier
  • Charles Ffrench-Constant
  • Robin J. M. Franklin
  • Dick Hoekstra
  • Chao Zhao
  • Wia Baron

Remyelination following central nervous system demyelination is essential to prevent axon degeneration. However, remyelination ultimately fails in demyelinating diseases such as multiple sclerosis. This failure of remyelination is likely mediated by many factors, including changes in the extracellular signalling environment. Here, we examined the expression of the extracellular matrix molecule fibronectin on demyelinating injury and how this affects remyelination by oligodendrocytes progenitors. In toxin-induced lesions undergoing efficient remyelination, fibronectin expression was transiently increased within demyelinated areas and declined as remyelination proceeded. Fibronectin levels increased both by leakage from the blood circulation and by production from central nervous system resident cells. In chronically demyelinated multiple sclerosis lesions, fibronectin expression persisted in the form of aggregates, which may render fibronectin resistant to degradation. Aggregation of fibronectin was similarly observed at the relapse phase of chronic experimental autoimmune encephalitis, but not on toxin-induced demyelination, suggesting that fibronectin aggregation is mediated by inflammation-induced demyelination. Indeed, the inflammatory mediator lipopolysaccharide induced fibronectin aggregation by astrocytes. Most intriguingly, injection of astrocyte-derived fibronectin aggregates in toxin-induced demyelinated lesions inhibited oligodendrocyte differentiation and remyelination, and fibronectin aggregates are barely expressed in remyelinated multiple sclerosis lesions. Therefore, these findings suggest that fibronectin aggregates within multiple sclerosis lesions contribute to remyelination failure. Hence, the inhibitory signals induced by fibronectin aggregates or factors that affect fibronectin aggregation could be potential therapeutic targets for promoting remyelination.

Original languageEnglish
Pages (from-to)116-131
Number of pages16
JournalBrain
Volume136
Publication statusPublished - Jan-2013

    Keywords

  • fibronectin, multiple sclerosis, remyelination, astrocyte, oligodendrocyte, CENTRAL-NERVOUS-SYSTEM, TOXIN-INDUCED DEMYELINATION, MYELIN MEMBRANE FORMATION, TOLL-LIKE RECEPTORS, EXTRACELLULAR-MATRIX, INCREASED EXPRESSION, EXPERIMENTAL-MODELS, CNS DEMYELINATION, RAFT-ASSOCIATION, PROGENITOR CELLS

ID: 5789685