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Feasibility of [18F]-RGD for ex vivo imaging of atherosclerosis in detection of alpha v beta 3 integrin expression

Golestani, R., Mirfeizi, L., Zeebregts, C. J., Westra, J., de Haas, H. J., Glaudemans, A. W. J. M., Koole, M., Luurtsema, G., Tio, R. A., Dierckx, R. A. J. O., Boersma, H. H., Elsinga, P. H. & Slart, R. H. J. A., Dec-2015, In : Journal of Nuclear Cardiology. 22, 6, p. 1179-1186 8 p.

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  • Feasibility of [18F]-RGD for ex vivo imaging of atherosclerosis in detection

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DOI

Background. Inflammation and angiogenesis play an important role in atherosclerotic plaque rupture. Therefore, molecular imaging of these processes could be used for determination of rupture-prone atherosclerotic plaques. alpha v beta 3 integrin is involved in the process of angiogenesis. Targeted imaging of alpha v beta 3 integrin has been shown to be possible in previous studies on tumor models, using radiolabeled arginine-glycine-aspartate (RGD). Our aim was to investigate feasibility of ex vivo detection of alpha v beta 3 integrin in carotid endarterectomy (CEA) specimens.

Methods and Results. Nineteen CEA specimens were incubated in 5 MBq [18F]-RGD-K5 for 1 hour followed by 1 hour emission microPET scan. The results were quantified in 4 mm wide segments as percent incubation dose per gram (%Inc/g). Segmental-to-total ratio was calculated and presence of alpha v beta 3 integrin and endothelial cells in each segment was confirmed by immunohistochemical staining for CD31 and alpha v beta 3 integrin, respectively. [18F]-RGD-K5 uptake was heterogeneously distributed across CEA specimens and was localized within the vessel wall. Significant correlations were observed between segmental-to-total ratio with alpha v beta 3 integrin staining score (r = 0.58, P = .038) and CD31 staining score (rho = 0.67, P <.002).

Conclusion. This study showed the feasibility of integrin imaging by determination of alpha v beta 3 integrin expression in human atherosclerotic plaques.

Original languageEnglish
Pages (from-to)1179-1186
Number of pages8
JournalJournal of Nuclear Cardiology
Volume22
Issue number6
Publication statusPublished - Dec-2015

    Keywords

  • MicroPET imaging, vulnerable atherosclerotic plaque, RGD, integrin, imaging, ex vivo, POSITRON-EMISSION-TOMOGRAPHY, CANCER-PATIENTS, ALPHA(V)BETA(3) EXPRESSION, RADIATION-DOSIMETRY, RGD PEPTIDES, PLAQUE, ANGIOGENESIS, VEGF, PET, F-18-GALACTO-RGD

ID: 16834299