Publication

Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans

Luteijn, M. J., van Bergeijk, P., Kaaij, L. J. T., Almeida, M. V., Roovers, E. F., Berezikov, E. & Ketting, R. F., 15-Aug-2012, In : The EMBO Journal. 31, 16, p. 3422-3430 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Luteijn, M. J., van Bergeijk, P., Kaaij, L. J. T., Almeida, M. V., Roovers, E. F., Berezikov, E., & Ketting, R. F. (2012). Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans. The EMBO Journal, 31(16), 3422-3430. https://doi.org/10.1038/emboj.2012.213

Author

Luteijn, Maartje J ; van Bergeijk, Petra ; Kaaij, Lucas J T ; Almeida, Miguel Vasconcelos ; Roovers, Elke F ; Berezikov, Eugene ; Ketting, René F. / Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans. In: The EMBO Journal. 2012 ; Vol. 31, No. 16. pp. 3422-3430.

Harvard

Luteijn, MJ, van Bergeijk, P, Kaaij, LJT, Almeida, MV, Roovers, EF, Berezikov, E & Ketting, RF 2012, 'Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans', The EMBO Journal, vol. 31, no. 16, pp. 3422-3430. https://doi.org/10.1038/emboj.2012.213

Standard

Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans. / Luteijn, Maartje J; van Bergeijk, Petra; Kaaij, Lucas J T; Almeida, Miguel Vasconcelos; Roovers, Elke F; Berezikov, Eugene; Ketting, René F.

In: The EMBO Journal, Vol. 31, No. 16, 15.08.2012, p. 3422-3430.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Luteijn MJ, van Bergeijk P, Kaaij LJT, Almeida MV, Roovers EF, Berezikov E et al. Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans. The EMBO Journal. 2012 Aug 15;31(16):3422-3430. https://doi.org/10.1038/emboj.2012.213


BibTeX

@article{e70dd7015cae46a39a87117183ac824c,
title = "Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans",
abstract = "In recent years, the Piwi pathway has been shown to regulate the silencing of mobile genetic elements. However, we know little about how Piwi pathways impose silencing and even less about trans-generational stability of Piwi-induced silencing. We demonstrate that the Caenorhabditis elegans Piwi protein PRG-1 can initiate an extremely stable form of gene silencing on a transgenic, single-copy target. This type of silencing is faithfully maintained over tens of generations in the absence of a functional Piwi pathway. Interestingly, RNAi can also trigger permanent gene silencing of a single-copy transgene and the phenomenon will be collectively referred to as RNA-induced epigenetic silencing (RNAe). RNAe can act in trans and is dependent on endogenous RNAi factors. The involvement of factors known to act in nuclear RNAi and the fact that RNAe is accompanied by repressive chromatin marks indicate that RNAe includes a transcriptional silencing component. Our results demonstrate that, at least in C. elegans, the Piwi pathway can impose a state of gene silencing that borders on 'permanently silent'. Such a property may be more widely conserved among Piwi pathways in different animals.",
keywords = "Animals, Animals, Genetically Modified, Argonaute Proteins, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Profiling, Gene Silencing, Models, Biological, Journal Article, Research Support, Non-U.S. Gov't",
author = "Luteijn, {Maartje J} and {van Bergeijk}, Petra and Kaaij, {Lucas J T} and Almeida, {Miguel Vasconcelos} and Roovers, {Elke F} and Eugene Berezikov and Ketting, {Ren{\'e} F}",
year = "2012",
month = aug,
day = "15",
doi = "10.1038/emboj.2012.213",
language = "English",
volume = "31",
pages = "3422--3430",
journal = "The EMBO Journal",
issn = "0261-4189",
publisher = "Wiley",
number = "16",

}

RIS

TY - JOUR

T1 - Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans

AU - Luteijn, Maartje J

AU - van Bergeijk, Petra

AU - Kaaij, Lucas J T

AU - Almeida, Miguel Vasconcelos

AU - Roovers, Elke F

AU - Berezikov, Eugene

AU - Ketting, René F

PY - 2012/8/15

Y1 - 2012/8/15

N2 - In recent years, the Piwi pathway has been shown to regulate the silencing of mobile genetic elements. However, we know little about how Piwi pathways impose silencing and even less about trans-generational stability of Piwi-induced silencing. We demonstrate that the Caenorhabditis elegans Piwi protein PRG-1 can initiate an extremely stable form of gene silencing on a transgenic, single-copy target. This type of silencing is faithfully maintained over tens of generations in the absence of a functional Piwi pathway. Interestingly, RNAi can also trigger permanent gene silencing of a single-copy transgene and the phenomenon will be collectively referred to as RNA-induced epigenetic silencing (RNAe). RNAe can act in trans and is dependent on endogenous RNAi factors. The involvement of factors known to act in nuclear RNAi and the fact that RNAe is accompanied by repressive chromatin marks indicate that RNAe includes a transcriptional silencing component. Our results demonstrate that, at least in C. elegans, the Piwi pathway can impose a state of gene silencing that borders on 'permanently silent'. Such a property may be more widely conserved among Piwi pathways in different animals.

AB - In recent years, the Piwi pathway has been shown to regulate the silencing of mobile genetic elements. However, we know little about how Piwi pathways impose silencing and even less about trans-generational stability of Piwi-induced silencing. We demonstrate that the Caenorhabditis elegans Piwi protein PRG-1 can initiate an extremely stable form of gene silencing on a transgenic, single-copy target. This type of silencing is faithfully maintained over tens of generations in the absence of a functional Piwi pathway. Interestingly, RNAi can also trigger permanent gene silencing of a single-copy transgene and the phenomenon will be collectively referred to as RNA-induced epigenetic silencing (RNAe). RNAe can act in trans and is dependent on endogenous RNAi factors. The involvement of factors known to act in nuclear RNAi and the fact that RNAe is accompanied by repressive chromatin marks indicate that RNAe includes a transcriptional silencing component. Our results demonstrate that, at least in C. elegans, the Piwi pathway can impose a state of gene silencing that borders on 'permanently silent'. Such a property may be more widely conserved among Piwi pathways in different animals.

KW - Animals

KW - Animals, Genetically Modified

KW - Argonaute Proteins

KW - Caenorhabditis elegans

KW - Caenorhabditis elegans Proteins

KW - Gene Expression Profiling

KW - Gene Silencing

KW - Models, Biological

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/emboj.2012.213

DO - 10.1038/emboj.2012.213

M3 - Article

C2 - 22850670

VL - 31

SP - 3422

EP - 3430

JO - The EMBO Journal

JF - The EMBO Journal

SN - 0261-4189

IS - 16

ER -

ID: 54915806