Publication

Expression pattern analysis of microRNAs in Caenorhabditis elegans

Isik, M. & Berezikov, E., 2013, In : Methods in Molecular Biology. 936, p. 129-141 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Isik, M., & Berezikov, E. (2013). Expression pattern analysis of microRNAs in Caenorhabditis elegans. Methods in Molecular Biology, 936, 129-141. https://doi.org/10.1007/978-1-62703-083-0_11

Author

Isik, Meltem ; Berezikov, Eugene. / Expression pattern analysis of microRNAs in Caenorhabditis elegans. In: Methods in Molecular Biology. 2013 ; Vol. 936. pp. 129-141.

Harvard

Isik, M & Berezikov, E 2013, 'Expression pattern analysis of microRNAs in Caenorhabditis elegans', Methods in Molecular Biology, vol. 936, pp. 129-141. https://doi.org/10.1007/978-1-62703-083-0_11

Standard

Expression pattern analysis of microRNAs in Caenorhabditis elegans. / Isik, Meltem; Berezikov, Eugene.

In: Methods in Molecular Biology, Vol. 936, 2013, p. 129-141.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Isik M, Berezikov E. Expression pattern analysis of microRNAs in Caenorhabditis elegans. Methods in Molecular Biology. 2013;936:129-141. https://doi.org/10.1007/978-1-62703-083-0_11


BibTeX

@article{2c1e5ca731634a88a0a3f2ac33e48380,
title = "Expression pattern analysis of microRNAs in Caenorhabditis elegans",
abstract = "MicroRNAs (miRNAs) are ∼22 nucleotide single-stranded RNA molecules that originate from hairpin precursors and regulate gene expression at the posttranscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. miRNAs play important roles in a variety of biological processes, including development, proliferation, differentiation, cell fate determination, apoptosis, signal transduction, host-viral interactions, and tumorigenesis. Methodological advances in miRNA studies allowed identification of biological roles for many miRNAs, and establishing the spatiotemporal expression patterns of miRNAs is one of the approaches to elucidate their biological functions. Expression pattern analysis of miRNAs helps to identify potential genetic interactors that exhibit similar expression patterns and this, combined with further supporting experiments, helps to identify the genetic pathways in which the specific miRNAs are involved. In this chapter, we describe a detailed protocol for the analysis of miRNA expression patterns in Caenorhabditis elegans.",
keywords = "Animals, Animals, Genetically Modified, Caenorhabditis elegans, Cloning, Molecular, Gene Expression Profiling, Gene Expression Regulation, MicroRNAs, Microscopy, Fluorescence, Promoter Regions, Genetic, Transcriptome, Journal Article",
author = "Meltem Isik and Eugene Berezikov",
year = "2013",
doi = "10.1007/978-1-62703-083-0_11",
language = "English",
volume = "936",
pages = "129--141",
journal = "Methods in Molecular Biology",
issn = "1064-3745",

}

RIS

TY - JOUR

T1 - Expression pattern analysis of microRNAs in Caenorhabditis elegans

AU - Isik, Meltem

AU - Berezikov, Eugene

PY - 2013

Y1 - 2013

N2 - MicroRNAs (miRNAs) are ∼22 nucleotide single-stranded RNA molecules that originate from hairpin precursors and regulate gene expression at the posttranscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. miRNAs play important roles in a variety of biological processes, including development, proliferation, differentiation, cell fate determination, apoptosis, signal transduction, host-viral interactions, and tumorigenesis. Methodological advances in miRNA studies allowed identification of biological roles for many miRNAs, and establishing the spatiotemporal expression patterns of miRNAs is one of the approaches to elucidate their biological functions. Expression pattern analysis of miRNAs helps to identify potential genetic interactors that exhibit similar expression patterns and this, combined with further supporting experiments, helps to identify the genetic pathways in which the specific miRNAs are involved. In this chapter, we describe a detailed protocol for the analysis of miRNA expression patterns in Caenorhabditis elegans.

AB - MicroRNAs (miRNAs) are ∼22 nucleotide single-stranded RNA molecules that originate from hairpin precursors and regulate gene expression at the posttranscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. miRNAs play important roles in a variety of biological processes, including development, proliferation, differentiation, cell fate determination, apoptosis, signal transduction, host-viral interactions, and tumorigenesis. Methodological advances in miRNA studies allowed identification of biological roles for many miRNAs, and establishing the spatiotemporal expression patterns of miRNAs is one of the approaches to elucidate their biological functions. Expression pattern analysis of miRNAs helps to identify potential genetic interactors that exhibit similar expression patterns and this, combined with further supporting experiments, helps to identify the genetic pathways in which the specific miRNAs are involved. In this chapter, we describe a detailed protocol for the analysis of miRNA expression patterns in Caenorhabditis elegans.

KW - Animals

KW - Animals, Genetically Modified

KW - Caenorhabditis elegans

KW - Cloning, Molecular

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - MicroRNAs

KW - Microscopy, Fluorescence

KW - Promoter Regions, Genetic

KW - Transcriptome

KW - Journal Article

U2 - 10.1007/978-1-62703-083-0_11

DO - 10.1007/978-1-62703-083-0_11

M3 - Article

C2 - 23007505

VL - 936

SP - 129

EP - 141

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 54915758