Publication

Expression of interleukin-1 beta in rat dorsal root ganglia

Copray, JCVM., Mantingh, N. V., Brouwer, N., Biber, K., Kust, BM., Liem, RSB., Huitinga, N. V., Tilders, FJH., Van Dam, AM. & Boddeke, HWGM., 30-Aug-2001, In : Journal of Neuroimmunology. 118, 2, p. 203-211 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • JCVM Copray
  • [No Value] Mantingh
  • N Brouwer
  • K Biber
  • BM Kust
  • RSB Liem
  • [No Value] Huitinga
  • FJH Tilders
  • AM Van Dam
  • HWGM Boddeke

The expression of interleukin-lp was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in Situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1 beta mRNA: about 80% of these DRG neurons actually appeared to produce the IL-lp protein at markedly varying levels. The expression of IL-1 beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1 beta immunopositive sensory neurons included most of the large calretinin-positive la afferents. but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 beta receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1 beta in the DRG neurons needs to be elucidated, but we speculate that IL-1 beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission. (C) 2001 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalJournal of Neuroimmunology
Volume118
Issue number2
Publication statusPublished - 30-Aug-2001

    Keywords

  • interleukin-1 beta, sensory neurons, type 1 interleukin-1 receptor, dorsal root ganglion, IMMUNODEFICIENCY-VIRUS TYPE-1, PRIMARY AFFERENT NEURONS, NERVOUS-SYSTEM, MESSENGER-RNA, SPINAL-CORD, IMMUNOREACTIVE INTERLEUKIN-1-BETA, RECEPTOR ANTAGONIST, HIPPOCAMPAL-NEURONS, MULTIPLE-SCLEROSIS, RAMIFIED MICROGLIA

ID: 3964243