Publication

Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3

de Jong, E. K., de Haas, A. H., Brouwer, N., van Weering, H. R. J., Hensens, M., Bechmann, I., Pratley, P., Wesseling, E., Boddeke, H. W. G. M. & Biber, K., Jun-2008, In : Journal of Neurochemistry. 105, 5, p. 1726-1736 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

de Jong, E. K., de Haas, A. H., Brouwer, N., van Weering, H. R. J., Hensens, M., Bechmann, I., ... Biber, K. (2008). Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3. Journal of Neurochemistry, 105(5), 1726-1736. https://doi.org/10.1111/j.1471-4159.2008.05267.x

Author

de Jong, Eiko K. ; de Haas, Alexander H. ; Brouwer, Nieske ; van Weering, Hilmar R. J. ; Hensens, Marjolein ; Bechmann, Ingo ; Pratley, Pierre ; Wesseling, Evelyn ; Boddeke, Hendrikus W. G. M. ; Biber, Knut. / Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3. In: Journal of Neurochemistry. 2008 ; Vol. 105, No. 5. pp. 1726-1736.

Harvard

de Jong, EK, de Haas, AH, Brouwer, N, van Weering, HRJ, Hensens, M, Bechmann, I, Pratley, P, Wesseling, E, Boddeke, HWGM & Biber, K 2008, 'Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3', Journal of Neurochemistry, vol. 105, no. 5, pp. 1726-1736. https://doi.org/10.1111/j.1471-4159.2008.05267.x

Standard

Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3. / de Jong, Eiko K.; de Haas, Alexander H.; Brouwer, Nieske; van Weering, Hilmar R. J.; Hensens, Marjolein; Bechmann, Ingo; Pratley, Pierre; Wesseling, Evelyn; Boddeke, Hendrikus W. G. M.; Biber, Knut.

In: Journal of Neurochemistry, Vol. 105, No. 5, 06.2008, p. 1726-1736.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

de Jong EK, de Haas AH, Brouwer N, van Weering HRJ, Hensens M, Bechmann I et al. Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3. Journal of Neurochemistry. 2008 Jun;105(5):1726-1736. https://doi.org/10.1111/j.1471-4159.2008.05267.x


BibTeX

@article{9a5290a53a96489f848d545e0e1d5122,
title = "Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3",
abstract = "Signaling through chemokine receptor CXCR3 in the brain has been implicated in various brain diseases, as CXCR3 and its ligands are found under these conditions. Recently, a new chemokine ligand for CXCR3 was reported. In humans, an alternatively spliced variant of CXCR3 expressed on microvascular endothelial cells, named CXCR3b, was shown to bind CXCL4. In the periphery, the cellular expression and functions of CXCL4 are well described but in the brain its expression and function are unknown. Here, we show that brain microglia are a cellular source of CXCL4 in vitro and in vivo under neurodegenerating conditions. Microglial migration induced by CXCL4 is absent in CXCR3-deficient microglia, indicating a role of CXCR3. CXCL4 furthermore attenuates lipopolysaccharide-induced microglial phagocytosis and nitric oxide production in microglia and BV-2 cells. Based on these findings, it is proposed that locally released CXCL4 may control microglia responses.",
keywords = "CNS, CXCL4, CXCR3, microglia, CENTRAL-NERVOUS-SYSTEM, EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS, INTERFERON-INDUCIBLE PROTEIN-10, CHEMOKINE RECEPTOR CXCR3, IFN-GAMMA, MULTIPLE-SCLEROSIS, MOLECULAR CHARACTERIZATION, LEUKOCYTE MIGRATION, CUTTING EDGE, ACTIVATION",
author = "{de Jong}, {Eiko K.} and {de Haas}, {Alexander H.} and Nieske Brouwer and {van Weering}, {Hilmar R. J.} and Marjolein Hensens and Ingo Bechmann and Pierre Pratley and Evelyn Wesseling and Boddeke, {Hendrikus W. G. M.} and Knut Biber",
year = "2008",
month = "6",
doi = "10.1111/j.1471-4159.2008.05267.x",
language = "English",
volume = "105",
pages = "1726--1736",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Blackwell Publishing Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3

AU - de Jong, Eiko K.

AU - de Haas, Alexander H.

AU - Brouwer, Nieske

AU - van Weering, Hilmar R. J.

AU - Hensens, Marjolein

AU - Bechmann, Ingo

AU - Pratley, Pierre

AU - Wesseling, Evelyn

AU - Boddeke, Hendrikus W. G. M.

AU - Biber, Knut

PY - 2008/6

Y1 - 2008/6

N2 - Signaling through chemokine receptor CXCR3 in the brain has been implicated in various brain diseases, as CXCR3 and its ligands are found under these conditions. Recently, a new chemokine ligand for CXCR3 was reported. In humans, an alternatively spliced variant of CXCR3 expressed on microvascular endothelial cells, named CXCR3b, was shown to bind CXCL4. In the periphery, the cellular expression and functions of CXCL4 are well described but in the brain its expression and function are unknown. Here, we show that brain microglia are a cellular source of CXCL4 in vitro and in vivo under neurodegenerating conditions. Microglial migration induced by CXCL4 is absent in CXCR3-deficient microglia, indicating a role of CXCR3. CXCL4 furthermore attenuates lipopolysaccharide-induced microglial phagocytosis and nitric oxide production in microglia and BV-2 cells. Based on these findings, it is proposed that locally released CXCL4 may control microglia responses.

AB - Signaling through chemokine receptor CXCR3 in the brain has been implicated in various brain diseases, as CXCR3 and its ligands are found under these conditions. Recently, a new chemokine ligand for CXCR3 was reported. In humans, an alternatively spliced variant of CXCR3 expressed on microvascular endothelial cells, named CXCR3b, was shown to bind CXCL4. In the periphery, the cellular expression and functions of CXCL4 are well described but in the brain its expression and function are unknown. Here, we show that brain microglia are a cellular source of CXCL4 in vitro and in vivo under neurodegenerating conditions. Microglial migration induced by CXCL4 is absent in CXCR3-deficient microglia, indicating a role of CXCR3. CXCL4 furthermore attenuates lipopolysaccharide-induced microglial phagocytosis and nitric oxide production in microglia and BV-2 cells. Based on these findings, it is proposed that locally released CXCL4 may control microglia responses.

KW - CNS

KW - CXCL4

KW - CXCR3

KW - microglia

KW - CENTRAL-NERVOUS-SYSTEM

KW - EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS

KW - INTERFERON-INDUCIBLE PROTEIN-10

KW - CHEMOKINE RECEPTOR CXCR3

KW - IFN-GAMMA

KW - MULTIPLE-SCLEROSIS

KW - MOLECULAR CHARACTERIZATION

KW - LEUKOCYTE MIGRATION

KW - CUTTING EDGE

KW - ACTIVATION

U2 - 10.1111/j.1471-4159.2008.05267.x

DO - 10.1111/j.1471-4159.2008.05267.x

M3 - Article

VL - 105

SP - 1726

EP - 1736

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 5

ER -

ID: 4723184