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Expressed protein profile of a Tectomicrobium and other microbial symbionts in the marine sponge Aplysina aerophoba as evidenced by metaproteomics

Chaib De Mares, M., Jiménez, D. J., Palladino, G., Gutleben, J., Lebrun, L. A., Muller, E. E. L., Wilmes, P., Sipkema, D. & van Elsas, J. D., 7-Aug-2018, In : Scientific Reports. 8, 14 p., 11795.

Research output: Contribution to journalArticleAcademicpeer-review

  • Maryam Chaib De Mares
  • Diego Javier Jiménez
  • Giorgia Palladino
  • Johanna Gutleben
  • Laura A. Lebrun
  • Emilie E.L. Muller
  • Paul Wilmes
  • Detmer Sipkema
  • Jan Dirk van Elsas
Aplysina aerophoba is an emerging model marine sponge, with a well-characterized microbial community in terms of diversity and structure. However, little is known about the expressed functional capabilities of its associated microbes. Here, we present the first metaproteomics-based study of the microbiome of A. aerophoba. We found that transport and degradation of halogenated and chloroaromatic compounds are common active processes in the sponge microbiomes. Our data further reveal that the highest number of proteins were affiliated to a sponge-associated Tectomicrobium, presumably from the family Entotheonellaceae, as well as to the well-known symbiont “Candidatus Synechococcus spongiarium”, suggesting a high metabolic activity of these two microorganisms in situ. Evidence for nitric oxide (NO) conversion to nitrous oxide was consistently observed for Tectomicrobia across replicates, by production of the NorQ protein. Moreover, we found a potential energy-yielding pathway through CO oxidation by putative Chloroflexi bacteria. Finally, we observed expression of enzymes that may be involved in the transformation of chitin, glycoproteins, glycolipids and glucans into smaller molecules, consistent with glycosyl hydrolases predicted from analyses of the genomes of Poribacteria sponge symbionts. Thus, this study provides crucial links between expressed proteins and specific members of the A. aerophoba microbiome.
Original languageEnglish
Article number11795
Number of pages14
JournalScientific Reports
Volume8
Publication statusPublished - 7-Aug-2018

    Keywords

  • SINGLE-CELL GENOMICS, SULFUR OXIDATION, WEB SERVER, BACTERIA, ABUNDANCE, DIVERSITY, SEQUENCES, COMMUNITY, REVEALS, MICROORGANISMS
Related Datasets
  1. Replication Data for: Expressed protein profile of a Tectomicrobium and other microbial symbionts in the marine sponge Aplysina aerophoba as evidenced by metaproteomics

    Chaib De Mares, M. (Creator), Jimenez, J. G. (Creator), Lebrun, L. A. (Creator), Muller, E. E. L. (Creator), Wilmes, P. (Creator), Sipkema, D. (Creator) & Elsas, van, J. D. (Creator), University of Groningen, 2018

    Dataset

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