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Exploiting the peptidoglycan-binding motif, LysM, for medical and industrial applications

Visweswaran, G. R. R., Leenhouts, K., van Roosmalen, M., Kok, J. & Buist, G., May-2014, In : Applied Microbiology and Biotechnology. 98, 10, p. 4331-4345 15 p.

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DOI

The lysin motif (LysM) was first identified by Garvey et al. in 1986 and, in subsequent studies, has been shown to bind noncovalently to peptidoglycan and chitin by interacting with N-acetylglucosamine moieties. The LysM sequence is present singly or repeatedly in a large number of proteins of prokaryotes and eukaryotes. Since the mid-1990s, domains containing one or more of these LysM sequences originating from different LysM-containing proteins have been examined for purely scientific reasons as well as for their possible use in various medical and industrial applications. These studies range from detecting localized binding of LysM-containing proteins onto bacteria to actual bacterial cell surface analysis. On a more applied level, the possibilities of employing the LysM domains for cell immobilization, for the display of peptides, proteins, or enzymes on (bacterial) surfaces as well as their utility in the development of novel vaccines have been scrutinized. To serve these purposes, the chimeric proteins containing one or more of the LysM sequences have been produced and isolated from various prokaryotic and eukaryotic expression hosts. This review gives a succinct overview of the characteristics of the LysM domain and of current developments in its application potential.

Original languageEnglish
Pages (from-to)4331-4345
Number of pages15
JournalApplied Microbiology and Biotechnology
Volume98
Issue number10
Publication statusPublished - May-2014

    Keywords

  • LysM, Noncovalent peptidoglycan binding, Vaccine, Cell immobilization, Protein display, Microbe detection, GRAM-POSITIVE BACTERIA, LACTIC-ACID BACTERIA, SURFACE DISPLAY SYSTEM, TERMINAL REPEAT REGION, CELL-WALL BINDING, LACTOCOCCUS-LACTIS, STAPHYLOCOCCUS-AUREUS, ESCHERICHIA-COLI, LACTOBACILLUS-PLANTARUM, NUCLEOTIDE-SEQUENCE

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