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Ex Situ Normothermic Machine Perfusion of Donor Livers

Karimian, N., Matton, A. P. M., Westerkamp, A. C., Burlage, L. C., Op den Dries, S., Leuvenink, H. G. D., Lisman, T., Uygun, K., Markmann, J. F. & Porte, R. J., May-2015, In : Journal of Visualized Experiments. 99, 9 p., 52688.

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  • Ex Situ Normothermic Machine Perfusion of Donor Livers

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DOI

In contrast to conventional static cold preservation (0-4 degrees C), ex situ machine perfusion may provide better preservation of donor livers. Continuous perfusion of organs provides the opportunity to improve organ quality and allows ex situ viability assessment of donor livers prior to transplantation. This video article provides a step by step protocol for ex situ normothermic machine perfusion (37 degrees C) of human donor livers using a device that provides a pressure and temperature controlled pulsatile perfusion of the hepatic artery and continuous perfusion of the portal vein. The perfusion fluid is oxygenated by two hollow fiber membrane oxygenators and the temperature can be regulated between 10 degrees C and 37 degrees C. During perfusion, the metabolic activity of the liver as well as the degree of injury can be assessed by biochemical analysis of samples taken from the perfusion fluid. Machine perfusion is a very promising tool to increase the number of livers that are suitable for transplantation.

Original languageEnglish
Article number52688
Number of pages9
JournalJournal of Visualized Experiments
Issue number99
Publication statusPublished - May-2015

    Keywords

  • Medicine, Issue 99, Machine perfusion, liver transplantation, preservation, normothermic, hypothermic, human donor liver, BILIARY COMPLICATIONS, RISK-FACTORS, TRANSPLANTATION, PRESERVATION, GRAFTS, REPERFUSION, STRICTURES, INJURY

ID: 24396673