Publication

Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology

Zadesenets, K. S., Vizoso, D. B., Schlatter, A., Konopatskaia, I. D., Berezikov, E., Scharer, L. & Rubtsov, N. B., 18-Oct-2016, In : PLoS ONE. 11, 10, 24 p., e0164915.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Zadesenets, K. S., Vizoso, D. B., Schlatter, A., Konopatskaia, I. D., Berezikov, E., Scharer, L., & Rubtsov, N. B. (2016). Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology. PLoS ONE, 11(10), [e0164915]. https://doi.org/10.1371/journal.pone.0164915

Author

Zadesenets, Kira S. ; Vizoso, Dita B. ; Schlatter, Aline ; Konopatskaia, Irina D. ; Berezikov, Eugene ; Scharer, Lukas ; Rubtsov, Nikolay B. / Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology. In: PLoS ONE. 2016 ; Vol. 11, No. 10.

Harvard

Zadesenets, KS, Vizoso, DB, Schlatter, A, Konopatskaia, ID, Berezikov, E, Scharer, L & Rubtsov, NB 2016, 'Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology', PLoS ONE, vol. 11, no. 10, e0164915. https://doi.org/10.1371/journal.pone.0164915

Standard

Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology. / Zadesenets, Kira S.; Vizoso, Dita B.; Schlatter, Aline; Konopatskaia, Irina D.; Berezikov, Eugene; Scharer, Lukas; Rubtsov, Nikolay B.

In: PLoS ONE, Vol. 11, No. 10, e0164915, 18.10.2016.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Zadesenets KS, Vizoso DB, Schlatter A, Konopatskaia ID, Berezikov E, Scharer L et al. Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology. PLoS ONE. 2016 Oct 18;11(10). e0164915. https://doi.org/10.1371/journal.pone.0164915


BibTeX

@article{a72d57ab98cf4531a86b890c5d860d20,
title = "Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology",
abstract = "Over the past decade, the free-living flatworm Macrostomum lignano has been successfully used in many areas of biology, including embryology, stem cells, sexual selection, bioadhesion and aging. The increased use of this powerful laboratory model, including the establishment of genomic resources and tools, makes it essential to have a detailed description of the chromosome organization of this species, previously suggested to have a karyotype with 2n = 8 and one pair of large and three pairs of small metacentric chromosomes. We performed cytogenetic analyses for chromosomes of one commonly used inbred line of M. lignano (called DV1) and uncovered unexpected chromosome number variation in the form of aneuploidies of the largest chromosomes. These results prompted us to perform karyotypic studies in individual specimens of this and other lines of M. lignano reared under laboratory conditions, as well as in freshly field-collected specimens from different natural populations. Our analyses revealed a high frequency of aneuploids and in some cases other numerical and structural chromosome abnormalities in laboratory-reared lines of M. lignano, and some cases of aneuploidy were also found in freshly field-collected specimens. Moreover, karyological analyses were performed in specimens of three further species: Macrostomum sp. 8 (a close relative of M. lignano), M. spirale and M. hystrix. Macrostomum sp. 8 showed a karyotype that was similar to that of M. lignano, with tetrasomy for its largest chromosome being the most common karyotype, while the other two species showed a simpler karyotype that is more typical of the genus Macrostomum. These findings suggest that M. lignano and Macrostomum sp. 8 can be used as new models for studying processes of partial genome duplication in genome evolution.",
keywords = "STEM-CELLS, SIMULTANEOUS HERMAPHRODITE, CHROMOSOMAL DUPLICATION, GENOME EVOLUTION, SEX ALLOCATION, DNA-SEQUENCES, REGENERATION, ANEUPLOIDY, PLATYHELMINTHES, POLYPLOIDY",
author = "Zadesenets, {Kira S.} and Vizoso, {Dita B.} and Aline Schlatter and Konopatskaia, {Irina D.} and Eugene Berezikov and Lukas Scharer and Rubtsov, {Nikolay B.}",
year = "2016",
month = oct,
day = "18",
doi = "10.1371/journal.pone.0164915",
language = "English",
volume = "11",
journal = "PLOS-One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "10",

}

RIS

TY - JOUR

T1 - Evidence for Karyotype Polymorphism in the Free-Living Flatworm, Macrostomum lignano, a Model Organism for Evolutionary and Developmental Biology

AU - Zadesenets, Kira S.

AU - Vizoso, Dita B.

AU - Schlatter, Aline

AU - Konopatskaia, Irina D.

AU - Berezikov, Eugene

AU - Scharer, Lukas

AU - Rubtsov, Nikolay B.

PY - 2016/10/18

Y1 - 2016/10/18

N2 - Over the past decade, the free-living flatworm Macrostomum lignano has been successfully used in many areas of biology, including embryology, stem cells, sexual selection, bioadhesion and aging. The increased use of this powerful laboratory model, including the establishment of genomic resources and tools, makes it essential to have a detailed description of the chromosome organization of this species, previously suggested to have a karyotype with 2n = 8 and one pair of large and three pairs of small metacentric chromosomes. We performed cytogenetic analyses for chromosomes of one commonly used inbred line of M. lignano (called DV1) and uncovered unexpected chromosome number variation in the form of aneuploidies of the largest chromosomes. These results prompted us to perform karyotypic studies in individual specimens of this and other lines of M. lignano reared under laboratory conditions, as well as in freshly field-collected specimens from different natural populations. Our analyses revealed a high frequency of aneuploids and in some cases other numerical and structural chromosome abnormalities in laboratory-reared lines of M. lignano, and some cases of aneuploidy were also found in freshly field-collected specimens. Moreover, karyological analyses were performed in specimens of three further species: Macrostomum sp. 8 (a close relative of M. lignano), M. spirale and M. hystrix. Macrostomum sp. 8 showed a karyotype that was similar to that of M. lignano, with tetrasomy for its largest chromosome being the most common karyotype, while the other two species showed a simpler karyotype that is more typical of the genus Macrostomum. These findings suggest that M. lignano and Macrostomum sp. 8 can be used as new models for studying processes of partial genome duplication in genome evolution.

AB - Over the past decade, the free-living flatworm Macrostomum lignano has been successfully used in many areas of biology, including embryology, stem cells, sexual selection, bioadhesion and aging. The increased use of this powerful laboratory model, including the establishment of genomic resources and tools, makes it essential to have a detailed description of the chromosome organization of this species, previously suggested to have a karyotype with 2n = 8 and one pair of large and three pairs of small metacentric chromosomes. We performed cytogenetic analyses for chromosomes of one commonly used inbred line of M. lignano (called DV1) and uncovered unexpected chromosome number variation in the form of aneuploidies of the largest chromosomes. These results prompted us to perform karyotypic studies in individual specimens of this and other lines of M. lignano reared under laboratory conditions, as well as in freshly field-collected specimens from different natural populations. Our analyses revealed a high frequency of aneuploids and in some cases other numerical and structural chromosome abnormalities in laboratory-reared lines of M. lignano, and some cases of aneuploidy were also found in freshly field-collected specimens. Moreover, karyological analyses were performed in specimens of three further species: Macrostomum sp. 8 (a close relative of M. lignano), M. spirale and M. hystrix. Macrostomum sp. 8 showed a karyotype that was similar to that of M. lignano, with tetrasomy for its largest chromosome being the most common karyotype, while the other two species showed a simpler karyotype that is more typical of the genus Macrostomum. These findings suggest that M. lignano and Macrostomum sp. 8 can be used as new models for studying processes of partial genome duplication in genome evolution.

KW - STEM-CELLS

KW - SIMULTANEOUS HERMAPHRODITE

KW - CHROMOSOMAL DUPLICATION

KW - GENOME EVOLUTION

KW - SEX ALLOCATION

KW - DNA-SEQUENCES

KW - REGENERATION

KW - ANEUPLOIDY

KW - PLATYHELMINTHES

KW - POLYPLOIDY

U2 - 10.1371/journal.pone.0164915

DO - 10.1371/journal.pone.0164915

M3 - Article

C2 - 27755577

VL - 11

JO - PLOS-One

JF - PLOS-One

SN - 1932-6203

IS - 10

M1 - e0164915

ER -

ID: 40319115