Publication

Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes

Geuverink, E., Kraaijeveld, K., van Leussen, M., Chen, F., Pijpe, J., Linskens, M. H. K., Beukeboom, L. W. & van de Zande, L., Dec-2018, In : Insect Molecular Biology. 27, 6, p. 780-795 16 p., imb.12522.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Geuverink, E., Kraaijeveld, K., van Leussen, M., Chen, F., Pijpe, J., Linskens, M. H. K., ... van de Zande, L. (2018). Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes. Insect Molecular Biology, 27(6), 780-795. [imb.12522]. https://doi.org/10.1111/imb.12522

Author

Geuverink, Elzemiek ; Kraaijeveld, Ken ; van Leussen, Marloes ; Chen, Fangying ; Pijpe, Jeroen ; Linskens, Maarten H K ; Beukeboom, Leo W ; van de Zande, Louis. / Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes. In: Insect Molecular Biology. 2018 ; Vol. 27, No. 6. pp. 780-795.

Harvard

Geuverink, E, Kraaijeveld, K, van Leussen, M, Chen, F, Pijpe, J, Linskens, MHK, Beukeboom, LW & van de Zande, L 2018, 'Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes', Insect Molecular Biology, vol. 27, no. 6, imb.12522, pp. 780-795. https://doi.org/10.1111/imb.12522

Standard

Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes. / Geuverink, Elzemiek; Kraaijeveld, Ken; van Leussen, Marloes; Chen, Fangying; Pijpe, Jeroen; Linskens, Maarten H K; Beukeboom, Leo W; van de Zande, Louis.

In: Insect Molecular Biology, Vol. 27, No. 6, imb.12522, 12.2018, p. 780-795.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Geuverink E, Kraaijeveld K, van Leussen M, Chen F, Pijpe J, Linskens MHK et al. Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes. Insect Molecular Biology. 2018 Dec;27(6):780-795. imb.12522. https://doi.org/10.1111/imb.12522


BibTeX

@article{308d9468f0924805801d374ff8c483fb,
title = "Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes",
abstract = "Transformer (tra) is the central gear in many insect sex determination pathways and transduces a wide range of primary signals. Mediated by transformer-2 (tra2) it directs sexual development into the female or male mode. Duplications of tra have been detected in numerous Hymenoptera, but a function in sex determination has been confirmed only in Apis mellifera. We identified a tra2 ortholog (Lc-tra2), a tra ortholog (Lc-tra) and a tra paralog (Lc-traB) in the genome of Leptopilina clavipes (Hymenoptera: Cynipidae). We compared the sequence and structural conservation of these genes between sexual (arrhenotokous) and asexual all-female producing (thelytokous) individuals. Lc-tra is sex-specifically spliced in adults consistent with its orthologous function. The male-specific regions of Lc-tra are conserved in both reproductive modes. The paralog Lc-traB lacks the genomic region coding for male-specific exons and can only be translated into a full length TRA-like peptide sequence. Furthermore, unlike LC-TRA, the LC-TRAB inter-strain sequence variation is not differentiated into a sexual and an asexual haplotype. The LC-TRAB protein interacts with LC-TRA as well as LC-TRA2. This suggests that Lc-traB functions as a conserved element in sex determination of sexual and asexual individuals. This article is protected by copyright. All rights reserved.",
author = "Elzemiek Geuverink and Ken Kraaijeveld and {van Leussen}, Marloes and Fangying Chen and Jeroen Pijpe and Linskens, {Maarten H K} and Beukeboom, {Leo W} and {van de Zande}, Louis",
note = "This article is protected by copyright. All rights reserved.",
year = "2018",
month = "12",
doi = "10.1111/imb.12522",
language = "English",
volume = "27",
pages = "780--795",
journal = "Insect Molecular Biology",
issn = "1365-2583",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - Evidence for involvement of a transformer paralog in sex determination of the wasp Leptopilina clavipes

AU - Geuverink, Elzemiek

AU - Kraaijeveld, Ken

AU - van Leussen, Marloes

AU - Chen, Fangying

AU - Pijpe, Jeroen

AU - Linskens, Maarten H K

AU - Beukeboom, Leo W

AU - van de Zande, Louis

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/12

Y1 - 2018/12

N2 - Transformer (tra) is the central gear in many insect sex determination pathways and transduces a wide range of primary signals. Mediated by transformer-2 (tra2) it directs sexual development into the female or male mode. Duplications of tra have been detected in numerous Hymenoptera, but a function in sex determination has been confirmed only in Apis mellifera. We identified a tra2 ortholog (Lc-tra2), a tra ortholog (Lc-tra) and a tra paralog (Lc-traB) in the genome of Leptopilina clavipes (Hymenoptera: Cynipidae). We compared the sequence and structural conservation of these genes between sexual (arrhenotokous) and asexual all-female producing (thelytokous) individuals. Lc-tra is sex-specifically spliced in adults consistent with its orthologous function. The male-specific regions of Lc-tra are conserved in both reproductive modes. The paralog Lc-traB lacks the genomic region coding for male-specific exons and can only be translated into a full length TRA-like peptide sequence. Furthermore, unlike LC-TRA, the LC-TRAB inter-strain sequence variation is not differentiated into a sexual and an asexual haplotype. The LC-TRAB protein interacts with LC-TRA as well as LC-TRA2. This suggests that Lc-traB functions as a conserved element in sex determination of sexual and asexual individuals. This article is protected by copyright. All rights reserved.

AB - Transformer (tra) is the central gear in many insect sex determination pathways and transduces a wide range of primary signals. Mediated by transformer-2 (tra2) it directs sexual development into the female or male mode. Duplications of tra have been detected in numerous Hymenoptera, but a function in sex determination has been confirmed only in Apis mellifera. We identified a tra2 ortholog (Lc-tra2), a tra ortholog (Lc-tra) and a tra paralog (Lc-traB) in the genome of Leptopilina clavipes (Hymenoptera: Cynipidae). We compared the sequence and structural conservation of these genes between sexual (arrhenotokous) and asexual all-female producing (thelytokous) individuals. Lc-tra is sex-specifically spliced in adults consistent with its orthologous function. The male-specific regions of Lc-tra are conserved in both reproductive modes. The paralog Lc-traB lacks the genomic region coding for male-specific exons and can only be translated into a full length TRA-like peptide sequence. Furthermore, unlike LC-TRA, the LC-TRAB inter-strain sequence variation is not differentiated into a sexual and an asexual haplotype. The LC-TRAB protein interacts with LC-TRA as well as LC-TRA2. This suggests that Lc-traB functions as a conserved element in sex determination of sexual and asexual individuals. This article is protected by copyright. All rights reserved.

U2 - 10.1111/imb.12522

DO - 10.1111/imb.12522

M3 - Article

C2 - 30039559

VL - 27

SP - 780

EP - 795

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 1365-2583

IS - 6

M1 - imb.12522

ER -

ID: 64274857