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Evaluating the antifibrotic potency of galunisertib in a human ex vivo model of liver fibrosis

Luangmonkong, T., Suriguga, S., Bigaeva, E., Boersema, M., Oosterhuis, D., de Jong, K. P., Schuppan, D., Mutsaers, H. A. M. & Olinga, P., Sep-2017, In : British Journal of Pharmacology. 174, 18, p. 3107-3117 11 p.

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  • Evaluating the antifibrotic potency of galunisertib in a human ex vivomodel of liver fibrosis

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Background and PurposeLiver fibrosis is a major cause of liver-related mortality and, so far, no effective antifibrotic drug is available. Galunisertib, a TGF- receptor type I kinase inhibitor, is a potential candidate for the treatment of liver fibrosis. Here, we evaluated the potency of galunisertib in a human ex vivo model of liver fibrosis.

Experimental ApproachAntifibrotic potency and associated mechanisms were studied ex vivo, using both healthy and cirrhotic human precision-cut liver slices. Fibrosis-related parameters, both transcriptional and translational level, were assessed after treatment with galunisertib.

Key ResultsGalunisertib showed a prominent antifibrotic potency. Phosphorylation of SMAD2 was inhibited, while that of SMAD1 remained unchanged. In healthy and cirrhotic human livers, spontaneous transcription of numerous genes encoding collagens, including collagen type I, 1, collagen maturation, non-collageneous extracellular matrix (ECM) components, ECM remodelling and selected ECM receptors was significantly decreased. The reduction of fibrosis-related transcription was paralleled by a significant inhibition of procollagen I C-peptide released by both healthy and cirrhotic human liver slices. Moreover, galunisertib showed similar antifibrotic potency in human and rat lives.

Conclusions and ImplicationsGalunisertib is a drug that deserves to be further investigated for the treatment of liver fibrosis. Inhibition of SMAD2 phosphorylation is probably a central mechanism of action. In addition, blocking the production and maturation of collagens and promoting their degradation are related to the antifibrotic action of galunisertib.

Original languageEnglish
Pages (from-to)3107-3117
Number of pages11
JournalBritish Journal of Pharmacology
Volume174
Issue number18
Early online date10-Jul-2017
Publication statusPublished - Sep-2017

    Keywords

  • GROWTH-FACTOR-BETA, PRECISION-CUT LIVER, HEPATIC STELLATE CELLS, COLLAGEN TYPE-I, LY2157299 MONOHYDRATE, SIGNALING PATHWAY, HEPATOCELLULAR-CARCINOMA, KINASE INHIBITOR, CONCISE GUIDE, EXPRESSION

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