Publication

Establishment of a chronic activity-based anorexia rat model

Frintrop, L., Trinh, S., Liesbrock, J., Paulukat, L., Kas, M. J., Tolba, R., Konrad, K., Herpertz-Dahlmann, B., Beyer, C. & Seitz, J., 1-Jan-2018, In : Journal of Neuroscience Methods. 293, p. 191-198 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Frintrop, L., Trinh, S., Liesbrock, J., Paulukat, L., Kas, M. J., Tolba, R., Konrad, K., Herpertz-Dahlmann, B., Beyer, C., & Seitz, J. (2018). Establishment of a chronic activity-based anorexia rat model. Journal of Neuroscience Methods, 293, 191-198. https://doi.org/10.1016/j.jneumeth.2017.09.018

Author

Frintrop, Linda ; Trinh, Stefanie ; Liesbrock, Johanna ; Paulukat, Lisa ; Kas, Martien J. ; Tolba, Rene ; Konrad, Kerstin ; Herpertz-Dahlmann, Beate ; Beyer, Cordian ; Seitz, Jochen. / Establishment of a chronic activity-based anorexia rat model. In: Journal of Neuroscience Methods. 2018 ; Vol. 293. pp. 191-198.

Harvard

Frintrop, L, Trinh, S, Liesbrock, J, Paulukat, L, Kas, MJ, Tolba, R, Konrad, K, Herpertz-Dahlmann, B, Beyer, C & Seitz, J 2018, 'Establishment of a chronic activity-based anorexia rat model', Journal of Neuroscience Methods, vol. 293, pp. 191-198. https://doi.org/10.1016/j.jneumeth.2017.09.018

Standard

Establishment of a chronic activity-based anorexia rat model. / Frintrop, Linda; Trinh, Stefanie; Liesbrock, Johanna; Paulukat, Lisa; Kas, Martien J.; Tolba, Rene; Konrad, Kerstin; Herpertz-Dahlmann, Beate; Beyer, Cordian; Seitz, Jochen.

In: Journal of Neuroscience Methods, Vol. 293, 01.01.2018, p. 191-198.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Frintrop L, Trinh S, Liesbrock J, Paulukat L, Kas MJ, Tolba R et al. Establishment of a chronic activity-based anorexia rat model. Journal of Neuroscience Methods. 2018 Jan 1;293:191-198. https://doi.org/10.1016/j.jneumeth.2017.09.018


BibTeX

@article{54e362392fb34c1383fc45a7b407df22,
title = "Establishment of a chronic activity-based anorexia rat model",
abstract = "AbstractBackground Anorexia nervosa (AN) is often a chronic eating disorder characterised by body image disturbance and low body weight often associated with starvation-induced amenorrhoea and excessive exercise. Activity-based anorexia (ABA) is an animal model representing many somatic aspects of this psychiatric illness. We systematically manipulated the extent and length of starvation and animal age to find the optimal parameters to study chronic starvation. New methods Wistar rats had 24 h/day running wheel access and received 40% of their baseline food intake until a 20% or 25% weight reduction was reached (acute starvation). This body weight was then maintained for two weeks (chronic starvation). The rats of different ages of 4 or 8 weeks were used to represent early and late adolescent animals, respectively. The complete absence of a menstrual cycle was defined as the primary outcome parameter. Results Acute starvation caused a disruption of the oestrous cycle in 58% of the animals. During chronic starvation, a complete loss of the oestrous cycle could be found. Furthermore, 4-week-old rats exhibited higher levels of hyperactivity and amenorrhoea than 8-week-old animals. A 20% starvation level led to 90% loss of cycle, while a 25% starvation level triggered complete loss. Comparison with existing methods Most current ABA models focus on acute starvation, while most patients are chronically ill. Conclusions The optimal parameters to achieve complete amenorrhoea included early adolescence, chronic starvation and 25% weight loss. The new ABA model allows studying the effects of chronic AN on underlying behavioural, hormonal and brain pathobiology.",
keywords = "Anorexia nervosa, Activity-based anorexia rat model, Acute vs. chronic starvation, Amenorrhoea, EXPRESSION, WEIGHT, CHRONIC FOOD RESTRICTION, EATING-DISORDERS, CALORIC RESTRICTION, DEHYDRATION-ANOREXIA, PHYSICAL-ACTIVITY, ESTROUS-CYCLE, FEMALE RATS, NERVOSA",
author = "Linda Frintrop and Stefanie Trinh and Johanna Liesbrock and Lisa Paulukat and Kas, {Martien J.} and Rene Tolba and Kerstin Konrad and Beate Herpertz-Dahlmann and Cordian Beyer and Jochen Seitz",
year = "2018",
month = jan,
day = "1",
doi = "10.1016/j.jneumeth.2017.09.018",
language = "English",
volume = "293",
pages = "191--198",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "ELSEVIER SCIENCE BV",

}

RIS

TY - JOUR

T1 - Establishment of a chronic activity-based anorexia rat model

AU - Frintrop, Linda

AU - Trinh, Stefanie

AU - Liesbrock, Johanna

AU - Paulukat, Lisa

AU - Kas, Martien J.

AU - Tolba, Rene

AU - Konrad, Kerstin

AU - Herpertz-Dahlmann, Beate

AU - Beyer, Cordian

AU - Seitz, Jochen

PY - 2018/1/1

Y1 - 2018/1/1

N2 - AbstractBackground Anorexia nervosa (AN) is often a chronic eating disorder characterised by body image disturbance and low body weight often associated with starvation-induced amenorrhoea and excessive exercise. Activity-based anorexia (ABA) is an animal model representing many somatic aspects of this psychiatric illness. We systematically manipulated the extent and length of starvation and animal age to find the optimal parameters to study chronic starvation. New methods Wistar rats had 24 h/day running wheel access and received 40% of their baseline food intake until a 20% or 25% weight reduction was reached (acute starvation). This body weight was then maintained for two weeks (chronic starvation). The rats of different ages of 4 or 8 weeks were used to represent early and late adolescent animals, respectively. The complete absence of a menstrual cycle was defined as the primary outcome parameter. Results Acute starvation caused a disruption of the oestrous cycle in 58% of the animals. During chronic starvation, a complete loss of the oestrous cycle could be found. Furthermore, 4-week-old rats exhibited higher levels of hyperactivity and amenorrhoea than 8-week-old animals. A 20% starvation level led to 90% loss of cycle, while a 25% starvation level triggered complete loss. Comparison with existing methods Most current ABA models focus on acute starvation, while most patients are chronically ill. Conclusions The optimal parameters to achieve complete amenorrhoea included early adolescence, chronic starvation and 25% weight loss. The new ABA model allows studying the effects of chronic AN on underlying behavioural, hormonal and brain pathobiology.

AB - AbstractBackground Anorexia nervosa (AN) is often a chronic eating disorder characterised by body image disturbance and low body weight often associated with starvation-induced amenorrhoea and excessive exercise. Activity-based anorexia (ABA) is an animal model representing many somatic aspects of this psychiatric illness. We systematically manipulated the extent and length of starvation and animal age to find the optimal parameters to study chronic starvation. New methods Wistar rats had 24 h/day running wheel access and received 40% of their baseline food intake until a 20% or 25% weight reduction was reached (acute starvation). This body weight was then maintained for two weeks (chronic starvation). The rats of different ages of 4 or 8 weeks were used to represent early and late adolescent animals, respectively. The complete absence of a menstrual cycle was defined as the primary outcome parameter. Results Acute starvation caused a disruption of the oestrous cycle in 58% of the animals. During chronic starvation, a complete loss of the oestrous cycle could be found. Furthermore, 4-week-old rats exhibited higher levels of hyperactivity and amenorrhoea than 8-week-old animals. A 20% starvation level led to 90% loss of cycle, while a 25% starvation level triggered complete loss. Comparison with existing methods Most current ABA models focus on acute starvation, while most patients are chronically ill. Conclusions The optimal parameters to achieve complete amenorrhoea included early adolescence, chronic starvation and 25% weight loss. The new ABA model allows studying the effects of chronic AN on underlying behavioural, hormonal and brain pathobiology.

KW - Anorexia nervosa

KW - Activity-based anorexia rat model

KW - Acute vs. chronic starvation

KW - Amenorrhoea

KW - EXPRESSION

KW - WEIGHT

KW - CHRONIC FOOD RESTRICTION

KW - EATING-DISORDERS

KW - CALORIC RESTRICTION

KW - DEHYDRATION-ANOREXIA

KW - PHYSICAL-ACTIVITY

KW - ESTROUS-CYCLE

KW - FEMALE RATS

KW - NERVOSA

U2 - 10.1016/j.jneumeth.2017.09.018

DO - 10.1016/j.jneumeth.2017.09.018

M3 - Article

VL - 293

SP - 191

EP - 198

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

ER -

ID: 48929104