Publication

Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo

Morrison, M., van der Heijden, R., Heeringa, P., Kaijzel, E., Verschuren, L., Blomhoff, R., Kooistra, T. & Kleemann, R., Mar-2014, In : ATHEROSCLEROSIS. 233, 1, p. 149-156 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Morrison, M., van der Heijden, R., Heeringa, P., Kaijzel, E., Verschuren, L., Blomhoff, R., ... Kleemann, R. (2014). Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo. ATHEROSCLEROSIS, 233(1), 149-156. https://doi.org/10.1016/j.atherosclerosis.2013.12.027

Author

Morrison, Martine ; van der Heijden, Roel ; Heeringa, Peter ; Kaijzel, Eric ; Verschuren, Lars ; Blomhoff, Rune ; Kooistra, Teake ; Kleemann, Robert. / Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo. In: ATHEROSCLEROSIS. 2014 ; Vol. 233, No. 1. pp. 149-156.

Harvard

Morrison, M, van der Heijden, R, Heeringa, P, Kaijzel, E, Verschuren, L, Blomhoff, R, Kooistra, T & Kleemann, R 2014, 'Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo', ATHEROSCLEROSIS, vol. 233, no. 1, pp. 149-156. https://doi.org/10.1016/j.atherosclerosis.2013.12.027

Standard

Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo. / Morrison, Martine; van der Heijden, Roel; Heeringa, Peter; Kaijzel, Eric; Verschuren, Lars; Blomhoff, Rune; Kooistra, Teake; Kleemann, Robert.

In: ATHEROSCLEROSIS, Vol. 233, No. 1, 03.2014, p. 149-156.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Morrison M, van der Heijden R, Heeringa P, Kaijzel E, Verschuren L, Blomhoff R et al. Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo. ATHEROSCLEROSIS. 2014 Mar;233(1):149-156. https://doi.org/10.1016/j.atherosclerosis.2013.12.027


BibTeX

@article{dc38a5b8d2d5423c9ba9cd8433e8b2bf,
title = "Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo",
abstract = "OBJECTIVE: Previous studies investigating flavanol-rich foods provide indications for potential cardioprotective effects of these foods, but the effects of individual flavanols remain unclear. We investigated whether the flavanol epicatechin can reduce diet-induced atherosclerosis, with particular emphasis on the cardiovascular risk factors dyslipidaemia and inflammation.METHODS: ApoE*3-Leiden mice were fed a cholesterol-containing atherogenic diet with or without epicatechin (0.1{\%} w/w) to study effects on early- and late-stage atherosclerosis (8 w and 20 w). In vivo effects of epicatechin on diet-induced inflammation were studied in human-CRP transgenic mice and NFκB-luciferase reporter mice.RESULTS: Epicatechin attenuated atherosclerotic lesion area in ApoE*3-Leiden mice by 27{\%}, without affecting plasma lipids. This anti-atherogenic effect of epicatechin was specific to the severe lesion types, with no effect on mild lesions. Epicatechin mitigated diet-induced increases in plasma SAA (in ApoE*3-Leiden mice) and plasma human-CRP (in human-CRP transgenic mice). Microarray analysis of aortic gene expression revealed an attenuating effect of epicatechin on several diet-induced pro-atherogenic inflammatory processes in the aorta (e.g. chemotaxis of cells, matrix remodelling), regulated by NFκB. These findings were confirmed immunohistochemically by reduced lesional neutrophil content in HCE, and by inhibition of diet-induced NFκB activity in epicatechin-treated NFκB-luciferase reporter mice.CONCLUSION: Epicatechin attenuates development of atherosclerosis and impairs lesion progression from mild to severe lesions in absence of an effect on dyslipidaemia. The observed reduction of circulating inflammatory risk factors by epicatechin (e.g. SAA, human-CRP), as well as its local anti-inflammatory activity in the vessel wall, provide a rationale for epicatechin's anti-atherogenic effects.",
keywords = "Atherosclerosis, CRP, Epicatechin, Inflammation, NF kappa B, Polyphenol, C-REACTIVE PROTEIN, CORONARY-HEART-DISEASE, SERUM-AMYLOID-A, CARDIOVASCULAR-DISEASE, MICE, RISK, INFLAMMATION, METAANALYSIS, CHOLESTEROL, CONSUMPTION",
author = "Martine Morrison and {van der Heijden}, Roel and Peter Heeringa and Eric Kaijzel and Lars Verschuren and Rune Blomhoff and Teake Kooistra and Robert Kleemann",
note = "Copyright {\circledC} 2014 Elsevier Ireland Ltd. All rights reserved.",
year = "2014",
month = "3",
doi = "10.1016/j.atherosclerosis.2013.12.027",
language = "English",
volume = "233",
pages = "149--156",
journal = "ATHEROSCLEROSIS",
issn = "0021-9150",
publisher = "ELSEVIER IRELAND LTD",
number = "1",

}

RIS

TY - JOUR

T1 - Epicatechin attenuates atherosclerosis and exerts anti-inflammatory effects on diet-induced human-CRP and NFκB in vivo

AU - Morrison, Martine

AU - van der Heijden, Roel

AU - Heeringa, Peter

AU - Kaijzel, Eric

AU - Verschuren, Lars

AU - Blomhoff, Rune

AU - Kooistra, Teake

AU - Kleemann, Robert

N1 - Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

PY - 2014/3

Y1 - 2014/3

N2 - OBJECTIVE: Previous studies investigating flavanol-rich foods provide indications for potential cardioprotective effects of these foods, but the effects of individual flavanols remain unclear. We investigated whether the flavanol epicatechin can reduce diet-induced atherosclerosis, with particular emphasis on the cardiovascular risk factors dyslipidaemia and inflammation.METHODS: ApoE*3-Leiden mice were fed a cholesterol-containing atherogenic diet with or without epicatechin (0.1% w/w) to study effects on early- and late-stage atherosclerosis (8 w and 20 w). In vivo effects of epicatechin on diet-induced inflammation were studied in human-CRP transgenic mice and NFκB-luciferase reporter mice.RESULTS: Epicatechin attenuated atherosclerotic lesion area in ApoE*3-Leiden mice by 27%, without affecting plasma lipids. This anti-atherogenic effect of epicatechin was specific to the severe lesion types, with no effect on mild lesions. Epicatechin mitigated diet-induced increases in plasma SAA (in ApoE*3-Leiden mice) and plasma human-CRP (in human-CRP transgenic mice). Microarray analysis of aortic gene expression revealed an attenuating effect of epicatechin on several diet-induced pro-atherogenic inflammatory processes in the aorta (e.g. chemotaxis of cells, matrix remodelling), regulated by NFκB. These findings were confirmed immunohistochemically by reduced lesional neutrophil content in HCE, and by inhibition of diet-induced NFκB activity in epicatechin-treated NFκB-luciferase reporter mice.CONCLUSION: Epicatechin attenuates development of atherosclerosis and impairs lesion progression from mild to severe lesions in absence of an effect on dyslipidaemia. The observed reduction of circulating inflammatory risk factors by epicatechin (e.g. SAA, human-CRP), as well as its local anti-inflammatory activity in the vessel wall, provide a rationale for epicatechin's anti-atherogenic effects.

AB - OBJECTIVE: Previous studies investigating flavanol-rich foods provide indications for potential cardioprotective effects of these foods, but the effects of individual flavanols remain unclear. We investigated whether the flavanol epicatechin can reduce diet-induced atherosclerosis, with particular emphasis on the cardiovascular risk factors dyslipidaemia and inflammation.METHODS: ApoE*3-Leiden mice were fed a cholesterol-containing atherogenic diet with or without epicatechin (0.1% w/w) to study effects on early- and late-stage atherosclerosis (8 w and 20 w). In vivo effects of epicatechin on diet-induced inflammation were studied in human-CRP transgenic mice and NFκB-luciferase reporter mice.RESULTS: Epicatechin attenuated atherosclerotic lesion area in ApoE*3-Leiden mice by 27%, without affecting plasma lipids. This anti-atherogenic effect of epicatechin was specific to the severe lesion types, with no effect on mild lesions. Epicatechin mitigated diet-induced increases in plasma SAA (in ApoE*3-Leiden mice) and plasma human-CRP (in human-CRP transgenic mice). Microarray analysis of aortic gene expression revealed an attenuating effect of epicatechin on several diet-induced pro-atherogenic inflammatory processes in the aorta (e.g. chemotaxis of cells, matrix remodelling), regulated by NFκB. These findings were confirmed immunohistochemically by reduced lesional neutrophil content in HCE, and by inhibition of diet-induced NFκB activity in epicatechin-treated NFκB-luciferase reporter mice.CONCLUSION: Epicatechin attenuates development of atherosclerosis and impairs lesion progression from mild to severe lesions in absence of an effect on dyslipidaemia. The observed reduction of circulating inflammatory risk factors by epicatechin (e.g. SAA, human-CRP), as well as its local anti-inflammatory activity in the vessel wall, provide a rationale for epicatechin's anti-atherogenic effects.

KW - Atherosclerosis

KW - CRP

KW - Epicatechin

KW - Inflammation

KW - NF kappa B

KW - Polyphenol

KW - C-REACTIVE PROTEIN

KW - CORONARY-HEART-DISEASE

KW - SERUM-AMYLOID-A

KW - CARDIOVASCULAR-DISEASE

KW - MICE

KW - RISK

KW - INFLAMMATION

KW - METAANALYSIS

KW - CHOLESTEROL

KW - CONSUMPTION

U2 - 10.1016/j.atherosclerosis.2013.12.027

DO - 10.1016/j.atherosclerosis.2013.12.027

M3 - Article

VL - 233

SP - 149

EP - 156

JO - ATHEROSCLEROSIS

JF - ATHEROSCLEROSIS

SN - 0021-9150

IS - 1

ER -

ID: 13707714