Enrichment of Cell Surface-Associated Proteins in Gram-Positive Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis

Bonn, F., Maaß, S. & van Dijl, J. M., 27-Sep-2018, Microbial Proteomics. Becher, D. (ed.). Humana Press, Vol. 1841. p. 35-43 9 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

In microbial cells surface-exposed proteins represent a physiologically important class of molecules as they enable these cells to interact with their environment both as free-living organisms and during interactions with a host. However, the characteristics of these proteins are quite divergent, which makes attempts to enrich, analyze, and quantify these molecules a challenging task. In this chapter two complementary methods for the enrichment and identification of cell surface-associated proteins, namely the biotinylation and the shaving approaches, are presented. Both protocols have been optimized for Gram-positive bacteria, and we provide a step-by-step guide for sample generation. Possible pitfalls during protein preparation are discussed.

Original languageEnglish
Title of host publicationMicrobial Proteomics
EditorsDörte Becher
PublisherHumana Press
Number of pages9
ISBN (Electronic)978-1-4939-8695-8
ISBN (Print)978-1-4939-8693-4
Publication statusE-pub ahead of print - 27-Sep-2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

View graph of relations

ID: 78183981