Enhancer of zeste homolog-2 (EZH2) methyltransferase regulates transgelin/smooth muscle-22 alpha expression in endothelial cells in response to interleukin-1 beta and transforming growth factor-beta 2Maleszewska, M., Gjaltema, R. A. F., Krenning, G. & Harmsen, M. C., Aug-2015, In : Cellular Signalling. 27, 8, p. 1589-1596 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Smooth muscle-22 alpha (SM22 alpha), encoded by transgelin (TAGLN), is expressed in mesenchymal lineage cells, including myofibroblasts and smooth muscle cells. It is an F-actin binding protein that regulates the organization of actin cytoskeleton, cellular contractility and motility. SM22 alpha is crucial for the maintenance of smooth muscle cell phenotype and its function. SM22 alpha is also expressed in the processes of mesenchymal transition of epithelial (EMT) or endothelial cells (EndMT). The expression of TAGLN/SM22 alpha is induced by transforming growth factor-beta (TGF beta) signaling and enhanced by concomitant interleukin-1 beta (IL-1 beta) signaling. We investigated the epigenetic regulation of TAGLN expression by enhancer of zeste homolog-2 (EZH2), the methyltransferase of Polycomb, in the context of TGF beta and IL-1 beta signaling in endothelial cells. We demonstrate that the expression of EZH2 in endothelial cells was regulated by the inflammatory cytokine IL-1 beta. A decrease in both expression and activity of EZH2 led to an increase in TAGLN expression. Inhibition of EZH2 augmented TGF beta 2-induced SM22 alpha expression. The decrease of EZH2 levels in endothelial cells co-stimulated with IL-1 beta and TGF beta 2 correlated with decreased H3K27me3 levels at the TAGLN proximal promoter. Moreover, the SM22 alpha expression increased. Taken together, this suggests that EZH2 regulates the chromatin structure at the TAGLN promoter through tri-methylation of H3K27. EZH2 therefore acts as an epigenetic integrator of IL-1 beta and TGF beta 2 signaling, providing an example of how cellular signaling can be resolved at the level of epigenetic regulation. Since IL-1 beta and TGF beta 2 represent the pro-inflammatory and pro-fibrotic conditions during vascular fibroproliferative disease, we surmise that EZH2, as the molecule that integrates their signaling, could also be a promising target for development of future therapy. (C) 2015 Elsevier Inc. All rights reserved.
|Number of pages||8|
|Publication status||Published - Aug-2015|
- Endothelial cell, EZH2, SM22 alpha, Inflammation, Fibrosis, Chromatin, VASCULAR SMOOTH-MUSCLE, TO-MESENCHYMAL TRANSITION, REPRESSIVE COMPLEX 2, GENE-EXPRESSION, IN-VITRO, LINKS INFLAMMATION, CARDIAC FIBROSIS, TARGET GENES, STEM-CELLS, SM22 ALPHA