Publication

Enhanced Bruton's tyrosine kinase in B-cells and autoreactive IgA in patients with idiopathic pulmonary fibrosis

Heukels, P., van Hulst, J. A. C., van Nimwegen, M., Boorsma, C. E., Melgert, B. N., von der Thusen, J. H., van den Blink, B., Hoek, R. A. S., Miedema, J. R., Neys, S. F. H., Corneth, O. B. J., Hendriks, R. W., Wijsenbeek, M. S. & Kool, M., 24-Oct-2019, In : Respiratory Research. 20, 1, 13 p., 232.

Research output: Contribution to journalArticleAcademicpeer-review

  • Peter Heukels
  • Jennifer A C van Hulst
  • Menno van Nimwegen
  • Carian E Boorsma
  • Barbro N Melgert
  • Jan H von der Thusen
  • Bernt van den Blink
  • Rogier A S Hoek
  • Jelle R Miedema
  • Stefan F H Neys
  • Odilia B J Corneth
  • Rudi W Hendriks
  • Marlies S Wijsenbeek
  • Mirjam Kool

Rationale Idiopathic Pulmonary Fibrosis (IPF) is thought to be triggered by repeated alveolar epithelial cell injury. Current evidence suggests that aberrant immune activation may contribute. However, the role of B-cell activation remains unclear. We determined the phenotype and activation status of B-cell subsets and evaluated the contribution of activated B-cells to the development of lung fibrosis both in humans and in mice. Methods B-cells in blood, mediastinal lymph node, and lung single-cell suspensions of IPF patients and healthy controls (HC) were characterized using 14-color flow cytometry. Mice were exposed to bleomycin to provoke pulmonary fibrosis. Results More IgA(+) memory B-cells and plasmablasts were found in blood (n = 27) and lungs (n = 11) of IPF patients compared to HC (n = 21) and control lungs (n = 9). IPF patients had higher levels of autoreactive IgA in plasma, which correlated with an enhanced decline of forced vital capacity (p = 0.002, r = - 0.50). Bruton's tyrosine kinase expression was higher in circulating IPF B-cells compared to HC, indicating enhanced B-cell activation. Bleomycin-exposed mice had increased pulmonary IgA(+) germinal center and plasma cell proportions compared to control mice. The degree of lung fibrosis correlated with pulmonary germinal center B-cell proportions (p = 0.010, r = 0.88). Conclusion Our study demonstrates that IPF patients have more circulating activated B-cells and autoreactive IgA, which correlate with disease progression. These B-cell alterations were also observed in the widely used mouse model of experimental pulmonary fibrosis. Autoreactive IgA could be useful as a biomarker for disease progression in IPF.

Original languageEnglish
Article number232
Number of pages13
JournalRespiratory Research
Volume20
Issue number1
Publication statusPublished - 24-Oct-2019

    Keywords

  • Idiopathic pulmonary fibrosis, B-cells, Auto-reactive IgA, Bruton's tyrosine kinase, Bleomycin, PROGNOSTIC BIOMARKER, IMMUNOGLOBULIN-A, DIFFERENTIATION, LYMPHOCYTES, RITUXIMAB, DIAGNOSIS, IMMUNITY, CXCL13

View graph of relations

Download statistics

No data available

ID: 100718137