Publication

Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products

Guzman-Chavez, F., Zwahlen, R. D., Bovenberg, R. A. L. & Driessen, A. J. M., 15-Nov-2018, In : Frontiers in Microbiology. 9, 25 p., 2768.

Research output: Contribution to journalReview articleAcademicpeer-review

APA

Guzman-Chavez, F., Zwahlen, R. D., Bovenberg, R. A. L., & Driessen, A. J. M. (2018). Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products. Frontiers in Microbiology, 9, [2768]. https://doi.org/10.3389/fmicb.2018.02768

Author

Guzman-Chavez, Fernando ; Zwahlen, Reto D. ; Bovenberg, Roel A. L. ; Driessen, Arnold J. M. / Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products. In: Frontiers in Microbiology. 2018 ; Vol. 9.

Harvard

Guzman-Chavez, F, Zwahlen, RD, Bovenberg, RAL & Driessen, AJM 2018, 'Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products', Frontiers in Microbiology, vol. 9, 2768. https://doi.org/10.3389/fmicb.2018.02768

Standard

Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products. / Guzman-Chavez, Fernando; Zwahlen, Reto D.; Bovenberg, Roel A. L.; Driessen, Arnold J. M.

In: Frontiers in Microbiology, Vol. 9, 2768, 15.11.2018.

Research output: Contribution to journalReview articleAcademicpeer-review

Vancouver

Guzman-Chavez F, Zwahlen RD, Bovenberg RAL, Driessen AJM. Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products. Frontiers in Microbiology. 2018 Nov 15;9. 2768. https://doi.org/10.3389/fmicb.2018.02768


BibTeX

@article{68e18135a31d43b1a2039ff0b8fee86b,
title = "Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products",
abstract = "Penicillium chrysogenum (renamed P. rubens) is the most studied member of a family of more than 350 Penicillium species that constitute the genus. Since the discovery of penicillin by Alexander Fleming, this filamentous fungus is used as a commercial beta-lactam antibiotic producer. For several decades, P. chrysogenum was subjected to a classical strain improvement (CSI) program to increase penicillin titers. This resulted in a massive increase in the penicillin production capacity, paralleled by the silencing of several other biosynthetic gene clusters (BGCs), causing a reduction in the production of a broad range of BGC encoded natural products (NPs). Several approaches have been used to restore the ability of the penicillin production strains to synthetize the NPs lost during the CSI. Here, we summarize various re-activation mechanisms of BGCs, and how interference with regulation can be used as a strategy to activate or silence BGCs in filamentous fungi. To further emphasize the versatility of P. chrysogenum as a fungal production platform for NPs with potential commercial value, protein engineering of biosynthetic enzymes is discussed as a tool to develop de novo BGC pathways for new NPs.",
keywords = "Penicillium chrysogenum, natural products, nonribosomal peptides, polyketides, gene activation, biosynthetic gene clusters, cell factory, NONRIBOSOMAL PEPTIDE SYNTHETASE, BIOSYNTHETIC GENE CLUSTERS, ACYL CARRIER PROTEIN, SECONDARY METABOLITE BIOSYNTHESIS, ISOPENICILLIN-N ACYLTRANSFERASE, MODULAR POLYKETIDE SYNTHASES, MBTH-LIKE PROTEINS, ASPERGILLUS-NIDULANS, TRANSCRIPTION FACTOR, CHEMICAL DIVERSITY",
author = "Fernando Guzman-Chavez and Zwahlen, {Reto D.} and Bovenberg, {Roel A. L.} and Driessen, {Arnold J. M.}",
year = "2018",
month = "11",
day = "15",
doi = "10.3389/fmicb.2018.02768",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Engineering of the Filamentous Fungus Penicillium chrysogenum as Cell Factory for Natural Products

AU - Guzman-Chavez, Fernando

AU - Zwahlen, Reto D.

AU - Bovenberg, Roel A. L.

AU - Driessen, Arnold J. M.

PY - 2018/11/15

Y1 - 2018/11/15

N2 - Penicillium chrysogenum (renamed P. rubens) is the most studied member of a family of more than 350 Penicillium species that constitute the genus. Since the discovery of penicillin by Alexander Fleming, this filamentous fungus is used as a commercial beta-lactam antibiotic producer. For several decades, P. chrysogenum was subjected to a classical strain improvement (CSI) program to increase penicillin titers. This resulted in a massive increase in the penicillin production capacity, paralleled by the silencing of several other biosynthetic gene clusters (BGCs), causing a reduction in the production of a broad range of BGC encoded natural products (NPs). Several approaches have been used to restore the ability of the penicillin production strains to synthetize the NPs lost during the CSI. Here, we summarize various re-activation mechanisms of BGCs, and how interference with regulation can be used as a strategy to activate or silence BGCs in filamentous fungi. To further emphasize the versatility of P. chrysogenum as a fungal production platform for NPs with potential commercial value, protein engineering of biosynthetic enzymes is discussed as a tool to develop de novo BGC pathways for new NPs.

AB - Penicillium chrysogenum (renamed P. rubens) is the most studied member of a family of more than 350 Penicillium species that constitute the genus. Since the discovery of penicillin by Alexander Fleming, this filamentous fungus is used as a commercial beta-lactam antibiotic producer. For several decades, P. chrysogenum was subjected to a classical strain improvement (CSI) program to increase penicillin titers. This resulted in a massive increase in the penicillin production capacity, paralleled by the silencing of several other biosynthetic gene clusters (BGCs), causing a reduction in the production of a broad range of BGC encoded natural products (NPs). Several approaches have been used to restore the ability of the penicillin production strains to synthetize the NPs lost during the CSI. Here, we summarize various re-activation mechanisms of BGCs, and how interference with regulation can be used as a strategy to activate or silence BGCs in filamentous fungi. To further emphasize the versatility of P. chrysogenum as a fungal production platform for NPs with potential commercial value, protein engineering of biosynthetic enzymes is discussed as a tool to develop de novo BGC pathways for new NPs.

KW - Penicillium chrysogenum

KW - natural products

KW - nonribosomal peptides

KW - polyketides

KW - gene activation

KW - biosynthetic gene clusters

KW - cell factory

KW - NONRIBOSOMAL PEPTIDE SYNTHETASE

KW - BIOSYNTHETIC GENE CLUSTERS

KW - ACYL CARRIER PROTEIN

KW - SECONDARY METABOLITE BIOSYNTHESIS

KW - ISOPENICILLIN-N ACYLTRANSFERASE

KW - MODULAR POLYKETIDE SYNTHASES

KW - MBTH-LIKE PROTEINS

KW - ASPERGILLUS-NIDULANS

KW - TRANSCRIPTION FACTOR

KW - CHEMICAL DIVERSITY

U2 - 10.3389/fmicb.2018.02768

DO - 10.3389/fmicb.2018.02768

M3 - Review article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 2768

ER -

ID: 71289735