Publication

Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications

Nijland, J. G. & Driessen, A. J. M., Jan-2020, In : Frontiers in Bioengineering and Biotechnology. 7, 13 p., 464.

Research output: Contribution to journalReview articleAcademicpeer-review

APA

Nijland, J. G., & Driessen, A. J. M. (2020). Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications. Frontiers in Bioengineering and Biotechnology, 7, [464]. https://doi.org/10.3389/fbioe.2019.00464

Author

Nijland, Jeroen G ; Driessen, Arnold J M. / Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications. In: Frontiers in Bioengineering and Biotechnology. 2020 ; Vol. 7.

Harvard

Nijland, JG & Driessen, AJM 2020, 'Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications', Frontiers in Bioengineering and Biotechnology, vol. 7, 464. https://doi.org/10.3389/fbioe.2019.00464

Standard

Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications. / Nijland, Jeroen G; Driessen, Arnold J M.

In: Frontiers in Bioengineering and Biotechnology, Vol. 7, 464, 01.2020.

Research output: Contribution to journalReview articleAcademicpeer-review

Vancouver

Nijland JG, Driessen AJM. Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications. Frontiers in Bioengineering and Biotechnology. 2020 Jan;7. 464. https://doi.org/10.3389/fbioe.2019.00464


BibTeX

@article{cbabb93483af4f698525eff19ee74e05,
title = "Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications",
abstract = "Lignocellulosic biomass yields after hydrolysis, besides the hexose D-glucose, D-xylose, and L-arabinose as main pentose sugars. In second generation bioethanol production utilizing the yeast Saccharomyces cerevisiae, it is critical that all three sugars are co-consumed to obtain an economically feasible and robust process. Since S. cerevisiae is unable to metabolize pentose sugars, metabolic pathway engineering has been employed to introduce the respective pathways for D-xylose and L-arabinose metabolism. However, S. cerevisiae lacks specific pentose transporters, and these sugars enter the cell with low affinity via glucose transporters of the Hxt family. Therefore, in the presence of D-glucose, utilization of D-xylose and L-arabinose is poor as the Hxt transporters prefer D-glucose. To solve this problem, heterologous expression of pentose transporters has been attempted but often with limited success due to poor expression and stability, and/or low turnover. A more successful approach is the engineering of the endogenous Hxt transporter family and evolutionary selection for D-glucose insensitive growth on pentose sugars. This has led to the identification of a critical and conserved asparagine residue in Hxt transporters that, when mutated, reduces the D-glucose affinity while leaving the D-xylose affinity mostly unaltered. Likewise, mutant Gal2 transporter have been selected supporting specific uptake of L-arabinose. In fermentation experiments, the transporter mutants support efficient uptake and consumption of pentose sugars, and even co-consumption of D-xylose and D-glucose when used at industrial concentrations. Further improvements are obtained by interfering with the post-translational inactivation of Hxt transporters at high or low D-glucose concentrations. Transporter engineering solved major limitations in pentose transport in yeast, now allowing for co-consumption of sugars that is limited only by the rates of primary metabolism. This paves the way for a more economical second-generation biofuels production process.",
keywords = "pentose transport, D-xylose, L-arabinose, yeast, bioethanol",
author = "Nijland, {Jeroen G} and Driessen, {Arnold J M}",
note = "Copyright {\textcopyright} 2020 Nijland and Driessen.",
year = "2020",
month = jan,
doi = "10.3389/fbioe.2019.00464",
language = "English",
volume = "7",
journal = "Frontiers in Bioengineering and Biotechnology",
issn = "2296-4185",
publisher = "Frontiers Media SA",

}

RIS

TY - JOUR

T1 - Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications

AU - Nijland, Jeroen G

AU - Driessen, Arnold J M

N1 - Copyright © 2020 Nijland and Driessen.

PY - 2020/1

Y1 - 2020/1

N2 - Lignocellulosic biomass yields after hydrolysis, besides the hexose D-glucose, D-xylose, and L-arabinose as main pentose sugars. In second generation bioethanol production utilizing the yeast Saccharomyces cerevisiae, it is critical that all three sugars are co-consumed to obtain an economically feasible and robust process. Since S. cerevisiae is unable to metabolize pentose sugars, metabolic pathway engineering has been employed to introduce the respective pathways for D-xylose and L-arabinose metabolism. However, S. cerevisiae lacks specific pentose transporters, and these sugars enter the cell with low affinity via glucose transporters of the Hxt family. Therefore, in the presence of D-glucose, utilization of D-xylose and L-arabinose is poor as the Hxt transporters prefer D-glucose. To solve this problem, heterologous expression of pentose transporters has been attempted but often with limited success due to poor expression and stability, and/or low turnover. A more successful approach is the engineering of the endogenous Hxt transporter family and evolutionary selection for D-glucose insensitive growth on pentose sugars. This has led to the identification of a critical and conserved asparagine residue in Hxt transporters that, when mutated, reduces the D-glucose affinity while leaving the D-xylose affinity mostly unaltered. Likewise, mutant Gal2 transporter have been selected supporting specific uptake of L-arabinose. In fermentation experiments, the transporter mutants support efficient uptake and consumption of pentose sugars, and even co-consumption of D-xylose and D-glucose when used at industrial concentrations. Further improvements are obtained by interfering with the post-translational inactivation of Hxt transporters at high or low D-glucose concentrations. Transporter engineering solved major limitations in pentose transport in yeast, now allowing for co-consumption of sugars that is limited only by the rates of primary metabolism. This paves the way for a more economical second-generation biofuels production process.

AB - Lignocellulosic biomass yields after hydrolysis, besides the hexose D-glucose, D-xylose, and L-arabinose as main pentose sugars. In second generation bioethanol production utilizing the yeast Saccharomyces cerevisiae, it is critical that all three sugars are co-consumed to obtain an economically feasible and robust process. Since S. cerevisiae is unable to metabolize pentose sugars, metabolic pathway engineering has been employed to introduce the respective pathways for D-xylose and L-arabinose metabolism. However, S. cerevisiae lacks specific pentose transporters, and these sugars enter the cell with low affinity via glucose transporters of the Hxt family. Therefore, in the presence of D-glucose, utilization of D-xylose and L-arabinose is poor as the Hxt transporters prefer D-glucose. To solve this problem, heterologous expression of pentose transporters has been attempted but often with limited success due to poor expression and stability, and/or low turnover. A more successful approach is the engineering of the endogenous Hxt transporter family and evolutionary selection for D-glucose insensitive growth on pentose sugars. This has led to the identification of a critical and conserved asparagine residue in Hxt transporters that, when mutated, reduces the D-glucose affinity while leaving the D-xylose affinity mostly unaltered. Likewise, mutant Gal2 transporter have been selected supporting specific uptake of L-arabinose. In fermentation experiments, the transporter mutants support efficient uptake and consumption of pentose sugars, and even co-consumption of D-xylose and D-glucose when used at industrial concentrations. Further improvements are obtained by interfering with the post-translational inactivation of Hxt transporters at high or low D-glucose concentrations. Transporter engineering solved major limitations in pentose transport in yeast, now allowing for co-consumption of sugars that is limited only by the rates of primary metabolism. This paves the way for a more economical second-generation biofuels production process.

KW - pentose transport

KW - D-xylose

KW - L-arabinose

KW - yeast

KW - bioethanol

U2 - 10.3389/fbioe.2019.00464

DO - 10.3389/fbioe.2019.00464

M3 - Review article

C2 - 32064252

VL - 7

JO - Frontiers in Bioengineering and Biotechnology

JF - Frontiers in Bioengineering and Biotechnology

SN - 2296-4185

M1 - 464

ER -

ID: 118411057