Engineering a nanopore with co-chaperonin function

Ho, C-W., Meervelt, V., Tsai, K-C., De Temmerman, P-J., Mast, J. & Maglia, G., 11-Dec-2015, In : Science Advances. 1, 11, 8 p., e1500905.

Research output: Contribution to journalArticleAcademicpeer-review

  • Ching-Wen Ho
  • Veerle Meervelt
  • Keng-Chang Tsai
  • Pieter-Jan De Temmerman
  • Jan Mast
  • Giovanni Maglia
The emergence of an enzymatic function can reveal functional insights and allows the engineering of biological systems with enhanced properties. We engineered an alpha hemolysin nanopore to function as GroES, a protein that, in complex with GroEL, forms a two-stroke protein-folding nanomachine. The transmembrane co-chaperonin was prepared by recombination of GroES functional elements with the nanopore, suggesting that emergent functions in molecular machines can be added bottom-up by incorporating modular elements into preexisting protein scaffolds. The binding of a single-ring version of GroEL to individual GroES nanopores prompted large changes to the unitary nanopore current, most likely reflecting the allosteric transitions of the chaperonin apical domains. One of the GroEL-induced current levels showed fast fluctuations (<1 ms), a characteristic that might be instrumental for efficient substrate encapsulation or folding. In the presence of unfolded proteins, the pattern of current transitions changed, suggesting a possible mechanism in which the free energy of adenosine triphosphate binding and hydrolysis is expended only when substrate proteins are occupied.
Original languageEnglish
Article numbere1500905
Number of pages8
JournalScience Advances
Issue number11
Publication statusPublished - 11-Dec-2015


  • single-molecule, protein folding, nanomachine, loop grafting, GroEL, GroES

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