Publication

Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia

van der Graaf, A. M., Wiegman, M. J., Plosch, T., Zeeman, G. G., van Buiten, A., Henning, R. H., Buikema, H. & Faas, M. M., 6-Nov-2013, In : PLoS ONE. 8, 11, 15 p., e79884.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van der Graaf, A. M., Wiegman, M. J., Plosch, T., Zeeman, G. G., van Buiten, A., Henning, R. H., ... Faas, M. M. (2013). Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia. PLoS ONE, 8(11), [e79884]. https://doi.org/10.1371/journal.pone.0079884

Author

van der Graaf, Anne Marijn ; Wiegman, Marjon J. ; Plosch, Torsten ; Zeeman, Gerda G. ; van Buiten, Azuwerus ; Henning, Robert H. ; Buikema, Hendrik ; Faas, Marijke M. / Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia. In: PLoS ONE. 2013 ; Vol. 8, No. 11.

Harvard

van der Graaf, AM, Wiegman, MJ, Plosch, T, Zeeman, GG, van Buiten, A, Henning, RH, Buikema, H & Faas, MM 2013, 'Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia', PLoS ONE, vol. 8, no. 11, e79884. https://doi.org/10.1371/journal.pone.0079884

Standard

Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia. / van der Graaf, Anne Marijn; Wiegman, Marjon J.; Plosch, Torsten; Zeeman, Gerda G.; van Buiten, Azuwerus; Henning, Robert H.; Buikema, Hendrik; Faas, Marijke M.

In: PLoS ONE, Vol. 8, No. 11, e79884, 06.11.2013.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van der Graaf AM, Wiegman MJ, Plosch T, Zeeman GG, van Buiten A, Henning RH et al. Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia. PLoS ONE. 2013 Nov 6;8(11). e79884. https://doi.org/10.1371/journal.pone.0079884


BibTeX

@article{63c55cadc4c04d6692e1173990f17bdb,
title = "Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia",
abstract = "Objective: We investigated endothelial dysfunction and the role of angiotensin (Ang)-II type I (AT1-R) and type II (AT2-R) receptor in the changes in the Ang-II sensitivity in experimental preeclampsia in the rat.Methods: Aortic rings were isolated from low dose lipopolysaccharide (LPS) infused pregnant rats (experimental preeclampsia; n=9), saline-infused pregnant rats (n=8), and saline (n=8) and LPS (n=8) infused non-pregnant rats. Endothelium-dependent acetylcholine--mediated relaxation was studied in phenylephrine-preconstricted aortic rings in the presence of vehicle, N-G-nitro-L-arginine methyl ester and/or indomethacin. To evaluate the role for AT1-R and AT2-R in Ang-II sensitivity, full concentration response curves were obtained for Ang-II in the presence of losartan or PD123319. mRNA expression of the AT1-R and AT2-R, eNOS and iNOS, COX1 and COX2 in aorta were evaluated using real-time RT-PCR.Results: The role of vasodilator prostaglandins in the aorta was increased and the role of endothelium-derived hyperpolarizing factor and response of the AT1-R and AT2-R to Ang-II was decreased in pregnant saline infused rats as compared with non-pregnant rats. These changes were not observed during preeclampsia.Conclusion: Pregnancy induced adaptations in endothelial function, which were not observed in the rat model for preeclampsia. This role of lack of pregnancy induced endothelial adaptation in the pathophysiology of experimental preeclampsia needs further investigation.",
keywords = "Angiotensin II, Angiotensin II Type 1 Receptor Blockers, Animals, Aorta, Cyclooxygenase 1, Cyclooxygenase 2, Disease Models, Animal, Endothelial Cells, Female, Imidazoles, Lipopolysaccharides, Losartan, Nitric Oxide Synthase Type II, Pre-Eclampsia, Pregnancy, Prostaglandins, Pyridines, Rats, Receptor, Angiotensin, Type 1, Receptor, Angiotensin, Type 2, Vasodilator Agents",
author = "{van der Graaf}, {Anne Marijn} and Wiegman, {Marjon J.} and Torsten Plosch and Zeeman, {Gerda G.} and {van Buiten}, Azuwerus and Henning, {Robert H.} and Hendrik Buikema and Faas, {Marijke M.}",
year = "2013",
month = "11",
day = "6",
doi = "10.1371/journal.pone.0079884",
language = "English",
volume = "8",
journal = "PLOS-One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "11",

}

RIS

TY - JOUR

T1 - Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia

AU - van der Graaf, Anne Marijn

AU - Wiegman, Marjon J.

AU - Plosch, Torsten

AU - Zeeman, Gerda G.

AU - van Buiten, Azuwerus

AU - Henning, Robert H.

AU - Buikema, Hendrik

AU - Faas, Marijke M.

PY - 2013/11/6

Y1 - 2013/11/6

N2 - Objective: We investigated endothelial dysfunction and the role of angiotensin (Ang)-II type I (AT1-R) and type II (AT2-R) receptor in the changes in the Ang-II sensitivity in experimental preeclampsia in the rat.Methods: Aortic rings were isolated from low dose lipopolysaccharide (LPS) infused pregnant rats (experimental preeclampsia; n=9), saline-infused pregnant rats (n=8), and saline (n=8) and LPS (n=8) infused non-pregnant rats. Endothelium-dependent acetylcholine--mediated relaxation was studied in phenylephrine-preconstricted aortic rings in the presence of vehicle, N-G-nitro-L-arginine methyl ester and/or indomethacin. To evaluate the role for AT1-R and AT2-R in Ang-II sensitivity, full concentration response curves were obtained for Ang-II in the presence of losartan or PD123319. mRNA expression of the AT1-R and AT2-R, eNOS and iNOS, COX1 and COX2 in aorta were evaluated using real-time RT-PCR.Results: The role of vasodilator prostaglandins in the aorta was increased and the role of endothelium-derived hyperpolarizing factor and response of the AT1-R and AT2-R to Ang-II was decreased in pregnant saline infused rats as compared with non-pregnant rats. These changes were not observed during preeclampsia.Conclusion: Pregnancy induced adaptations in endothelial function, which were not observed in the rat model for preeclampsia. This role of lack of pregnancy induced endothelial adaptation in the pathophysiology of experimental preeclampsia needs further investigation.

AB - Objective: We investigated endothelial dysfunction and the role of angiotensin (Ang)-II type I (AT1-R) and type II (AT2-R) receptor in the changes in the Ang-II sensitivity in experimental preeclampsia in the rat.Methods: Aortic rings were isolated from low dose lipopolysaccharide (LPS) infused pregnant rats (experimental preeclampsia; n=9), saline-infused pregnant rats (n=8), and saline (n=8) and LPS (n=8) infused non-pregnant rats. Endothelium-dependent acetylcholine--mediated relaxation was studied in phenylephrine-preconstricted aortic rings in the presence of vehicle, N-G-nitro-L-arginine methyl ester and/or indomethacin. To evaluate the role for AT1-R and AT2-R in Ang-II sensitivity, full concentration response curves were obtained for Ang-II in the presence of losartan or PD123319. mRNA expression of the AT1-R and AT2-R, eNOS and iNOS, COX1 and COX2 in aorta were evaluated using real-time RT-PCR.Results: The role of vasodilator prostaglandins in the aorta was increased and the role of endothelium-derived hyperpolarizing factor and response of the AT1-R and AT2-R to Ang-II was decreased in pregnant saline infused rats as compared with non-pregnant rats. These changes were not observed during preeclampsia.Conclusion: Pregnancy induced adaptations in endothelial function, which were not observed in the rat model for preeclampsia. This role of lack of pregnancy induced endothelial adaptation in the pathophysiology of experimental preeclampsia needs further investigation.

KW - Angiotensin II

KW - Angiotensin II Type 1 Receptor Blockers

KW - Animals

KW - Aorta

KW - Cyclooxygenase 1

KW - Cyclooxygenase 2

KW - Disease Models, Animal

KW - Endothelial Cells

KW - Female

KW - Imidazoles

KW - Lipopolysaccharides

KW - Losartan

KW - Nitric Oxide Synthase Type II

KW - Pre-Eclampsia

KW - Pregnancy

KW - Prostaglandins

KW - Pyridines

KW - Rats

KW - Receptor, Angiotensin, Type 1

KW - Receptor, Angiotensin, Type 2

KW - Vasodilator Agents

U2 - 10.1371/journal.pone.0079884

DO - 10.1371/journal.pone.0079884

M3 - Article

C2 - 24223202

VL - 8

JO - PLOS-One

JF - PLOS-One

SN - 1932-6203

IS - 11

M1 - e79884

ER -

ID: 5987276