Endothelial progenitor cells give rise to pro-angiogenic smooth muscle-like progenyMoonen, J-R. A. J., Krenning, G., Brinker, M. G. L., Koerts, J. A., van Luyn, M. J. A. & Harmsen, M. C., 1-Jun-2010, In : Cardiovascular Research. 86, 3, p. 506-515 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
Reciprocal plasticity exists between endothelial and mesenchymal lineages. For instance, mature endothelial cells adopt a smooth muscle-like phenotype through transforming growth factor beta-1 (TGF beta 1)-driven endothelial-to-mesenchymal transdifferentiation (EndMT). Peripheral blood contains circulating endothelial progenitor cells of which the endothelial colony-forming cells (ECFCs) harbour stem cell-like properties. Given the plasticity between endothelial and mesenchymal lineages and the stem cell-like properties of ECFCs, we hypothesized that ECFCs can give rise to smooth muscle-like progeny.
ECFCs were stimulated with TGF beta 1, after which TGF beta signalling cascades and their downstream effects were investigated. Indeed, EndMT of ECFCs resulted in smooth muscle-like progeniture. TGF beta 1-driven EndMT is mediated by ALK5 kinase activity, increased downstream Smad2 signalling, and reduced protein levels of inhibitor of DNA-binding protein 3. ECFCs lost expression of endothelial markers and endothelial anti-thrombogenic function. Simultaneously, mesenchymal marker expression was gained, cytoskeletal rearrangements occurred, and cells acquired a contractile phenotype. Transdifferentiated ECFCs were phenotypically stable and self-sustaining and, importantly, showed fibroblast growth factor-2 and angiopoietin-1-mediated pro-angiogenic paracrine properties.
Our study is the first to demonstrate that ECFCs can give rise to smooth muscle-like progeny, with potential therapeutic benefits. These findings further illustrate that ECFCs are highly plastic, which by itself has implications for therapeutical use.
|Number of pages||10|
|Publication status||Published - 1-Jun-2010|
- Endothelial progenitor cells, Plasticity, Transdifferentiation, Smooth muscle cells, EndMT, GROWTH-FACTOR-BETA, TO-MESENCHYMAL TRANSITION, EMBRYONIC STEM-CELLS, IN-VITRO, INDUCED RECRUITMENT, CORD BLOOD, TGF-BETA, PDGF-B, DIFFERENTIATION, VIVO