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Endo-glucanase digestion of oat beta-Glucan enhances Dectin-1 activation in human dendritic cells

Sahasrabudhe, N. M., Tian, L., van den Berg, M., Bruggeman, G., Bruininx, E., Schols, H. A., Faas, M. M. & de Vos, P., Mar-2016, In : Journal of Functional Foods. 21, p. 104-112 9 p.

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  • Endo-glucanase digestion of oat beta-Glucan enhances Dectin-1 activation

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DOI

Oat beta-Glucans were studied for their immunological impact before and after enzymatic digestion in order to enhance the efficacy of oat beta-Glucans for application in functional foods. Oat beta-Glucan is reported to have minimal impact compared to its fungal counterpart in vitro. Digestion with endo-glucanase enhanced its efficacy towards stimulating MCP-1, RANTES, IL-8, and IL-4 production in human dendritic cells as compared to the nondigested beta-Glucan. This effect resulted from an enhanced activation of the Dectin-1 receptor. Our data suggest that the immune-stimulation was dependent on the beta-(1-3) linkages and the reduced particle size of digested beta-Glucans. Thus, we show that enzymatic pre-digestion of dietary fibres such as oat beta-Glucan enhances its impact on specific immune receptors. We also demonstrate that particle size and/or molecular weight of oat beta-Glucans and exposure of specific binding sites for the receptors might be important tools for designing efficacious functional feed and food additives. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)104-112
Number of pages9
JournalJournal of Functional Foods
Volume21
Publication statusPublished - Mar-2016

    Keywords

  • Oat, beta-(1-3,1-4) Glucan, Dectin-1, Particle size, Endo-glucanase, Dendritic cells, INTESTINAL EPITHELIAL-CELLS, CHAIN FATTY-ACIDS, IN-VITRO, RECEPTOR, INFECTION, MACROPHAGES, DIETARY, MICE, IDENTIFICATION, RECOGNITION

ID: 30913714