Publication

Elg1 forms an alternative RFC complex important for DNA replication and genome integrity

Bellaoui, M., Chang, M., Ou, J., Xu, H., Boone, C. & Brown, G. W., 15-Aug-2003, In : EMBO Journal. 22, 16, p. 4304-4313 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Mohammed Bellaoui
  • Michael Chang
  • Jiongwen Ou
  • Hong Xu
  • Charles Boone
  • Grant W Brown

Genome-wide synthetic genetic interaction screens with mutants in the mus81 and mms4 replication fork-processing genes identified a novel replication factor C (RFC) homolog, Elg1, which forms an alternative RFC complex with Rfc2-5. This complex is distinct from the DNA replication RFC, the DNA damage checkpoint RFC and the sister chromatid cohesion RFC. As expected from its genetic interactions, elg1 mutants are sensitive to DNA damage. Elg1 is redundant with Rad24 in the DNA damage response and contributes to activation of the checkpoint kinase Rad53. We find that elg1 mutants display DNA replication defects and genome instability, including increased recombination and mutation frequencies, and minichromosome maintenance defects. Mutants in elg1 show genetic interactions with pathways required for processing of stalled replication forks, and are defective in recovery from DNA damage during S phase. We propose that Elg1-RFC functions both in normal DNA replication and in the DNA damage response.

Original languageEnglish
Pages (from-to)4304-4313
Number of pages10
JournalEMBO Journal
Volume22
Issue number16
Publication statusPublished - 15-Aug-2003

    Keywords

  • Carrier Proteins, Cell Cycle Proteins, Checkpoint Kinase 2, DNA Damage, DNA Replication, DNA, Fungal, DNA-Binding Proteins, Gene Deletion, Gene Expression Regulation, Fungal, Genes, Fungal, Genome, Hydroxyurea, Mutagens, Mutation, Nucleic Acid Synthesis Inhibitors, Oxygenases, Protein-Serine-Threonine Kinases, Recombination, Genetic, Replication Protein C, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ultraviolet Rays

ID: 14317282