Publication

Elevator-type mechanisms of membrane transport

Garaeva, A. A. & Slotboom, D. J., Jul-2020, In : Biochemical Society Transactions. 48, 3, p. 1227-1241 15 p., BST20200290.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

DOI

Membrane transporters are integral membrane proteins that mediate the passage of solutes across lipid bilayers. These proteins undergo conformational transitions between outward- and inward-facing states, which lead to alternating access of the substrate-binding site to the aqueous environment on either side of the membrane. Dozens of different transporter families have evolved, providing a wide variety of structural solutions to achieve alternating access. A sub-set of structurally diverse transporters operate by mechanisms that are collectively named 'elevator-type'. These transporters have one common characteristic: they contain a distinct protein domain that slides across the membrane as a rigid body, and in doing so it 'drags" the transported substrate along. Analysis of the global conformational changes that take place in membrane transporters using elevator-type mechanisms reveals that elevator-type movements can be achieved in more than one way. Molecular dynamics simulations and experimental data help to understand how lipid bilayer properties may affect elevator movements and vice versa.

Original languageEnglish
Article numberBST20200290
Pages (from-to)1227-1241
Number of pages15
JournalBiochemical Society Transactions
Volume48
Issue number3
Early online date2020
Publication statusPublished - Jul-2020

    Keywords

  • membrane proteins, protein structure, transport, AMINO-ACID TRANSPORTERS, SUBSTRATE-BINDING SITE, CRYSTAL-STRUCTURE, STRUCTURAL BIOLOGY, BACTERIAL HOMOLOG, S-COMPONENT, GLUTAMATE, TRANSLOCATION, INSIGHTS, ANTIPORTER

Download statistics

No data available

ID: 124809216