Elevator-type mechanisms of membrane transportGaraeva, A. A. & Slotboom, D. J., Jun-2020, In : Biochemical Society Transactions. 48, 3, p. 1227-1241 15 p., BST20200290.
Research output: Contribution to journal › Review article › Academic › peer-review
Membrane transporters are integral membrane proteins that mediate the passage of solutes across lipid bilayers. These proteins undergo conformational transitions between outward- and inward-facing states, which lead to alternating access of the substrate-binding site to the aqueous environment on either side of the membrane. Dozens of different transporter families have evolved, providing a wide variety of structural solutions to achieve alternating access. A sub-set of structurally diverse transporters operate by mechanisms that are collectively named 'elevator-type'. These transporters have one common characteristic: they contain a distinct protein domain that slides across the membrane as a rigid body, and in doing so it 'drags" the transported substrate along. Analysis of the global conformational changes that take place in membrane transporters using elevator-type mechanisms reveals that elevator-type movements can be achieved in more than one way. Molecular dynamics simulations and experimental data help to understand how lipid bilayer properties may affect elevator movements and vice versa.
|Number of pages||15|
|Journal||Biochemical Society Transactions|
|Early online date||2020|
|Publication status||Published - Jun-2020|
- membrane proteins, protein structure, transport, AMINO-ACID TRANSPORTERS, SUBSTRATE-BINDING SITE, CRYSTAL-STRUCTURE, STRUCTURAL BIOLOGY, BACTERIAL HOMOLOG, S-COMPONENT, GLUTAMATE, TRANSLOCATION, INSIGHTS, ANTIPORTER