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Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores

Huang, G., Willems, K., Soskine, M., Wloka, C. & Maglia, G., 16-Oct-2017, In : Nature Communications. 8, p. 1-11 11 p., 935.

Research output: Contribution to journalArticleAcademicpeer-review

Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and long read-length DNA sequencing applications. The analysis and sequencing of proteins, however, is complicated by their folded structure and non-uniform charge. Here we show that an electro-osmotic flow through Fragaceatoxin C (FraC) nanopores can be engineered to allow the entry of polypeptides at a fixed potential regardless of the charge composition of the polypeptide. We further use the nanopore currents to discriminate peptide and protein biomarkers from 25 kDa down to 1.3 kDa including polypeptides differing by one amino acid. On the road to nanopore proteomics, our findings represent a rationale for amino-acid analysis of folded and unfolded polypeptides with nanopores.Biological nanopore-based protein sequencing and recognition is challenging due to the folded structure or non-uniform charge of peptides. Here the authors show that engineered FraC nanopores can overcome these problems and recognize biomarkers in the form of oligopeptides, polypeptides and folded proteins.

Original languageEnglish
Article number935
Pages (from-to)1-11
Number of pages11
JournalNature Communications
Volume8
Publication statusPublished - 16-Oct-2017

    Keywords

  • Journal Article, SOLID-STATE NANOPORES, ALPHA-HEMOLYSIN NANOPORE, SINGLE-STRANDED-DNA, REAL-TIME DETECTION, BIOLOGICAL NANOPORE, MOLECULE DETECTION, MEMBRANE CHANNEL, GLASS NANOPORES, CLYA NANOPORE, PK(A) VALUES

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