Publication

Efficient recovery of environmental DNA for expression cloning by indirect extraction methods

Gabor, E., de Vries, E. & Janssen, DB., 15-May-2003, In : FEMS Microbial Ecology. 44, 2, p. 153-163 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Gabor, E., de Vries, E., & Janssen, DB. (2003). Efficient recovery of environmental DNA for expression cloning by indirect extraction methods. FEMS Microbial Ecology, 44(2), 153-163. https://doi.org/10.1016/S0168-6496(02)00462-2

Author

Gabor, Esther ; de Vries, Erik ; Janssen, DB. / Efficient recovery of environmental DNA for expression cloning by indirect extraction methods. In: FEMS Microbial Ecology. 2003 ; Vol. 44, No. 2. pp. 153-163.

Harvard

Gabor, E, de Vries, E & Janssen, DB 2003, 'Efficient recovery of environmental DNA for expression cloning by indirect extraction methods', FEMS Microbial Ecology, vol. 44, no. 2, pp. 153-163. https://doi.org/10.1016/S0168-6496(02)00462-2

Standard

Efficient recovery of environmental DNA for expression cloning by indirect extraction methods. / Gabor, Esther; de Vries, Erik; Janssen, DB.

In: FEMS Microbial Ecology, Vol. 44, No. 2, 15.05.2003, p. 153-163.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Gabor E, de Vries E, Janssen DB. Efficient recovery of environmental DNA for expression cloning by indirect extraction methods. FEMS Microbial Ecology. 2003 May 15;44(2):153-163. https://doi.org/10.1016/S0168-6496(02)00462-2


BibTeX

@article{6f5daf794b864ab293cac6b6d6f17cc5,
title = "Efficient recovery of environmental DNA for expression cloning by indirect extraction methods",
abstract = "Using direct and cell extraction-based (indirect) isolation methods, DNA was obtained from environmental samples with largely differing characteristics (loam soil, sand soil, sediment, activated sludge, and compost) and evaluated with respect to the comprised bacterial diversity and its suitability for expression cloning in Escherichia coli. Indirect DNA extraction methods yielded 10 to 100-fold lower amounts of DNA than direct procedures, but the bacterial diversity of DNA recovered by indirect means was distinctly higher as shown by denaturing gradient gel electrophoresis. Furthermore, much lower amounts of eukaryotic DNA were co-extracted if cell extraction-based methods were used (",
keywords = "expression cloning, denaturing gradient gel electrophoresis, environmental gene bank, cell extraction, direct DNA isolation, GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, POLYMERASE-CHAIN-REACTION, MICROBIAL DIVERSITY, ESCHERICHIA-COLI, BACTERIAL COMMUNITY, SEDIMENT SAMPLES, GENOMIC DNA, SOIL, LIBRARIES",
author = "Esther Gabor and {de Vries}, Erik and DB Janssen",
year = "2003",
month = "5",
day = "15",
doi = "10.1016/S0168-6496(02)00462-2",
language = "English",
volume = "44",
pages = "153--163",
journal = "FEMS Microbial Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Efficient recovery of environmental DNA for expression cloning by indirect extraction methods

AU - Gabor, Esther

AU - de Vries, Erik

AU - Janssen, DB

PY - 2003/5/15

Y1 - 2003/5/15

N2 - Using direct and cell extraction-based (indirect) isolation methods, DNA was obtained from environmental samples with largely differing characteristics (loam soil, sand soil, sediment, activated sludge, and compost) and evaluated with respect to the comprised bacterial diversity and its suitability for expression cloning in Escherichia coli. Indirect DNA extraction methods yielded 10 to 100-fold lower amounts of DNA than direct procedures, but the bacterial diversity of DNA recovered by indirect means was distinctly higher as shown by denaturing gradient gel electrophoresis. Furthermore, much lower amounts of eukaryotic DNA were co-extracted if cell extraction-based methods were used (

AB - Using direct and cell extraction-based (indirect) isolation methods, DNA was obtained from environmental samples with largely differing characteristics (loam soil, sand soil, sediment, activated sludge, and compost) and evaluated with respect to the comprised bacterial diversity and its suitability for expression cloning in Escherichia coli. Indirect DNA extraction methods yielded 10 to 100-fold lower amounts of DNA than direct procedures, but the bacterial diversity of DNA recovered by indirect means was distinctly higher as shown by denaturing gradient gel electrophoresis. Furthermore, much lower amounts of eukaryotic DNA were co-extracted if cell extraction-based methods were used (

KW - expression cloning

KW - denaturing gradient gel electrophoresis

KW - environmental gene bank

KW - cell extraction

KW - direct DNA isolation

KW - GRADIENT GEL-ELECTROPHORESIS

KW - 16S RIBOSOMAL-RNA

KW - POLYMERASE-CHAIN-REACTION

KW - MICROBIAL DIVERSITY

KW - ESCHERICHIA-COLI

KW - BACTERIAL COMMUNITY

KW - SEDIMENT SAMPLES

KW - GENOMIC DNA

KW - SOIL

KW - LIBRARIES

U2 - 10.1016/S0168-6496(02)00462-2

DO - 10.1016/S0168-6496(02)00462-2

M3 - Article

VL - 44

SP - 153

EP - 163

JO - FEMS Microbial Ecology

JF - FEMS Microbial Ecology

SN - 0168-6496

IS - 2

ER -

ID: 4096449