Publication

Efficient differentiation of CD14(+) monocytic cells into endothelial cells on degradable biomaterials

Krenning, G., Dankers, P. Y. W., Jovanovic, D., van Luyn, M. J. A. & Harmsen, M. C., Mar-2007, In : Biomaterials. 28, 8, p. 1470-1479 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

Vascular tissue engineering aims at creating self-renewing, anti-thrombogenic, vascular grafts, which can be based on endothelial progenitor cells (EPC). EPC harbor essential features such as plasticity and longevity. Unfortunately, the archetype CD34(+) EPC is rare in peripheral blood. Monocytes, i.e. CD14(+) cells also have the ability to differentiate into endothelial-like cells and are by far more abundant in peripheral blood than are CD34(+) EPC. Therefore, CD14(+) cells would seem appropriate candidates for tissue engineering of small-diameter blood vessels. In this study, we investigated the differentiation of CD14(+) cells on three biodegradable biomaterials under angiogenic conditions. Morphological analyses, gene transcript analyses, endothelial marker (i.e. VE-Cadherin and eNOS) and macrophage marker (i.e. CD68 and CD163) expression analyses, revealed that a small fraction (15-25%) of cultured CD14(+) cells differentiated into macrophages after 21 days of culture. The majority of CD14(+) cells (> 75%) differentiated into endothelial-like cells (ELC) on all biomaterials used. The expression of endothelial markers was similar to their expression on HUVEC. Since CD14(+) cells are present in high numbers in adult peripheral blood, easy to isolate and because they easily differentiate into ELC on biomaterials, we conclude that CD14(+) cells are a suitable cell source for progenitor-based vascular tissue engineering. (c) 2006 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1470-1479
Number of pages10
JournalBiomaterials
Volume28
Issue number8
Publication statusPublished - Mar-2007

    Keywords

  • cell culture, endothelial cell, monotype, polycaprolactone, polyurethane, RGD peptide, ANGIOGENIC GROWTH-FACTORS, PROGENITOR CELLS, STEM-CELLS, VASCULAR GRAFT, INTEGRINS, BIODEGRADATION, VASCULOGENESIS, EXPANSION, MARKERS, SYSTEMS

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