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EFFECTS ON BACILLUS-SUBTILIS OF A CONDITIONAL-LETHAL MUTATION IN THE ESSENTIAL GTP-BINDING PROTEIN OBG

KOK, J., TRACH, KA. & HOCH, JA., Dec-1994, In : Journal of Bacteriology. 176, 23, p. 7155-7160 6 p.

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  • Effects on Bacillus subtilis of a Conditional Lethal Mutation in the Essential GTP-Binding Protein Obg

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DOI

The obg gene is part of the spo0B sporulation operon and codes for a GTP-binding protein which is essential for growth. A temperature-sensitive mutant in the obg gene was isolated and found to be the result of two closely linked missense mutations in the amino domain of Obg. Temperature shift experiments revealed that the mutant was able to continue cell division for 2 to 3 generations at the nonpermissive temperature. Such experiments carried out during sporulation showed that Obg aas necessary for the transition from vegetative growth to stage 0 or stage II of sporulation, but sporulation subsequent to these stages was unaffected at the nonpermissive temperature. Spores of the temperature-sensitive mutant germinated normally at the nonpermissive temperature but failed to outgrow. The primary consequence of the obg mutation may be an alteration in initiation of chromosome replication.

Original languageEnglish
Pages (from-to)7155-7160
Number of pages6
JournalJournal of Bacteriology
Volume176
Issue number23
Publication statusPublished - Dec-1994

    Keywords

  • ESCHERICHIA-COLI, SPORULATION, GENE, ERA, SPO0A, PHOSPHORELAY, INITIATION, SEQUENCE, BACTERIA, GROWTH

ID: 121736126