Publication

Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.)

van Overbeek, L. & van Elsas, J. D., May-2008, In : FEMS Microbial Ecology. 64, 2, p. 283-296 14 p.

Research output: Contribution to journalArticleAcademicpeer-review

The effects of genotype, plant growth and experimental factors (soil and year) on potato-associated bacterial communities were studied. Cultivars Achirana Inta, Desiree, Merkur and transgenic Desiree line DL12 (containing T4 lysozyme gene) were assessed in two field experiments. Cross-comparisons between both experiments were made using Desiree plants. Culture-dependent and -independent approaches were used to demonstrate effects on total bacterial, actinobacterial and Pseudomonas communities in bulk and rhizosphere soils and endospheres. PCR-denaturing gradient gel electrophoresis fingerprints prepared with group-specific primers were analyzed using multivariate analyses and revealed that bacterial communities in Achirana Inta plants differed most from those of Desiree and Merkur. No significant effects were found between Desiree and DL12 lines. Plant growth stage strongly affected different plant-associated communities in both experiments. To investigate the effect of plant-associated communities on plant health, 800 isolates from rhizospheres and endospheres at the flowering stage were tested for suppression of Ralstonia solanacearum biovar 2 and/or Rhizoctonia solani AG3. A group of isolates closely resembling Lysobacter sp. dominated in young plants. Its prevalence was affected by plant growth stage and experiment rather than by plant genotype. It was concluded that plant growth stage overwhelmed any effect of plant genotype on the bacterial communities associated with potato.

Original languageEnglish
Pages (from-to)283-296
Number of pages14
JournalFEMS Microbial Ecology
Volume64
Issue number2
Publication statusPublished - May-2008

    Keywords

  • genetically modified plants, rhizosphere, endophytes, bacterial communities, cultivar, plant growth stage, GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, DAMPING-OFF DISEASE, TRANSGENIC POTATOES, RHIZOSPHERE BACTERIA, PSEUDOMONAS-FLUORESCENS, MICROBIAL-POPULATIONS, SYSTEMIC RESISTANCE, SERRATIA-PLYMUTHICA, BIOLOGICAL-CONTROL

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