Publication

Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat

van der Hoeven, JAB., Ter Horst, GJ., Molema, G., de Vos, P., Girbes, ARJ., Postema, F., Freund, RL., Wiersema, J., van Schilfgaarde, R. & Ploeg, RJ., Dec-2000, In : Annals of Surgery. 232, 6, p. 804-812 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

Objective

To assess the effect on the function and immunologic status of potential donor livers of the duration of brain death combined with the presence and absence of hemodynamic instability in the donor.

Summary Background Data

Brain death, regarded as a given condition in organ transplantation, could have significant effects on the donor organ quality.

Methods

Brain death was induced in Wistar rats. Short or long periods of brain death in the presence or absence of hemodynamic instability were applied. Sham-operated rats served as controls. Organ function was studied by monitoring standard serum parameters. The inflammatory status of the liver was assessed by determining the immediate early gene products, the expression of cell adhesion molecules, and the influx of leukocytes in the liver.

Results

Progressive organ dysfunction was most pronounced in hemodynamically unstable brain-dead donors. Irrespective of hemodynamic status, a progressive inflammatory activation could be observed in brain-dead rats compared with controls.

Conclusions

Brain death causes progressive liver dysfunction, which is made worse by the coexistence of hemodynamic instability. Further, brain death activates the inflammatory status of the potential donor liver. irrespective of the presence of hypotension. The changes observed may predispose the graft to additional damage from ischemia and reperfusion in the transplant procedure.

Original languageEnglish
Pages (from-to)804-812
Number of pages9
JournalAnnals of Surgery
Volume232
Issue number6
Publication statusPublished - Dec-2000
Event7th Annual Meeting of the European-Surgical-Association - , Netherlands
Duration: 14-Apr-200015-Apr-2000

Event

7th Annual Meeting of the European-Surgical-Association

14/04/200015/04/2000

Netherlands

Event: Other

    Keywords

  • ADHESION MOLECULES, KIDNEY-TRANSPLANTS, LIGAND MOLECULES, GRAFT FUNCTION, RISK-FACTORS, REJECTION, EXPRESSION, IMPACT, CATECHOLAMINES, DYSFUNCTION

ID: 3905172