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Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

Ronda, O. A. H. O., van de Heijning, B. J. M., Martini, I., Gerding, A., Wolters, J. C., van der Veen, Y. T., Koehorst, M., Jurdzinski, A., Havinga, R., van der Beek, E. M., Kuipers, F. & Verkade, H. J., 30-Sep-2020, In : Scientific Reports. 10, 1, 14 p., 16128.

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We recently reported that feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) results in lower body weights and fat mass gain upon high fat feeding in later life, compared to control (cIMF). To understand the underlying mechanisms, we now explored parameters possibly involved in this long- term effect. Male C57BL/6JOlaHsd mice, fed rodent diets containing eIMF or cIMF from postnatal (PN) day 16-42, were sacrificed at PN42. Hepatic proteins were measured using targeted proteomics. Lipids were assessed by LC-MS/MS (acylcarnitines) and GC-FID (fatty-acyl chain profiles). Early life growth and body composition, cytokines, and parameters of bile acid metabolism were similar between the groups. Hepatic concentrations of multiple proteins involved in beta-oxidation (+ 17%) the TCA cycle (+ 15%) and mitochondrial antioxidative proteins (+ 28%) were significantly higher in eIMF versus cIMF-fed mice (p <0.05). Hepatic l-carnitine levels, required for fatty acid uptake into the mitochondria, were higher (+ 33%, p <0.01) in eIMF-fed mice. The present study indicates that eIMF-fed mice have higher hepatic levels of proteins involved in fatty acid metabolism and oxidation. We speculate that eIMF feeding programs the metabolic handling of dietary lipids.

Original languageEnglish
Article number16128
Number of pages14
JournalScientific Reports
Volume10
Issue number1
Publication statusPublished - 30-Sep-2020

    Keywords

  • BODY-FAT ACCUMULATION, ADIPOSE-TISSUE, ACID OXIDATION, BREAST-MILK, OBESITY, LIVER, SIZE, LEPTIN, MOUSE, DEFICIENCY

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