Publication

Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA

de Groot, M., Leuvenink, HGD., Fekken, S., Schuurs, TA., van Schilfgaarde, R. & KEIZER, J., 1-Nov-2003, In : Journal of Biomedical Materials Research. Part A. 67A, 2, p. 679-683 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

de Groot, M., Leuvenink, HGD., Fekken, S., Schuurs, TA., van Schilfgaarde, R., & KEIZER, J. (2003). Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA. Journal of Biomedical Materials Research. Part A, 67A(2), 679-683. https://doi.org/10.1002/jbm.a.10023

Author

de Groot, M ; Leuvenink, HGD ; Fekken, S ; Schuurs, TA ; van Schilfgaarde, R ; KEIZER, J. / Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA. In: Journal of Biomedical Materials Research. Part A. 2003 ; Vol. 67A, No. 2. pp. 679-683.

Harvard

de Groot, M, Leuvenink, HGD, Fekken, S, Schuurs, TA, van Schilfgaarde, R & KEIZER, J 2003, 'Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA', Journal of Biomedical Materials Research. Part A, vol. 67A, no. 2, pp. 679-683. https://doi.org/10.1002/jbm.a.10023

Standard

Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA. / de Groot, M; Leuvenink, HGD; Fekken, S; Schuurs, TA; van Schilfgaarde, R; KEIZER, J.

In: Journal of Biomedical Materials Research. Part A, Vol. 67A, No. 2, 01.11.2003, p. 679-683.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

de Groot M, Leuvenink HGD, Fekken S, Schuurs TA, van Schilfgaarde R, KEIZER J. Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA. Journal of Biomedical Materials Research. Part A. 2003 Nov 1;67A(2):679-683. https://doi.org/10.1002/jbm.a.10023


BibTeX

@article{b8d68f97a99048bea30cab8a988ce351,
title = "Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA",
abstract = "Although the transplantation of alginate-poly-L-lysine-alginate encapsulated islets of Langerhans usually is successful, graft survival is still limited. Molecular analysis by RT-PCR of the encapsulated islets may provide insight into the mechanisms that affect islets during graft failure. However, RT-PCR on encapsulated islets is not possible because the poly-L-lysine of the capsule interferes with both cDNA synthesis and PCR amplification. We applied a method that mechanically removes the microcapsules from the islets after a short trypsin-EDTA treatment (decapsulation), thereby enabling RT-PCR analysis. The results of this study show that the decapsulation procedure does not affect islet vitality and has only minor effects on islet function and morphology. The decapsulation does not affect GAPDH, beta-actin, Bcl-2, or Bax gene expression. This method is an improvement over the time-consuming manual dissection of microcapsules because it allows for the rapid and relatively harmless removal of capsules on a larger scale. Decapsulation offers the possibility of applying RT-PCR, as well as other methods, which cannot be performed on encapsulated islets. (C) 2003 Wiley Periodicals, Inc.",
keywords = "alginate-poly-L-lysine-alginate microcapsules, islets of Langerhans, trypsin, EDTA, RT-PCR, PROPIDIUM IODIDE, ACRIDINE-ORANGE, BIOCOMPATIBILITY, VIABILITY, GRAFTS, CELLS",
author = "{de Groot}, M and HGD Leuvenink and S Fekken and TA Schuurs and {van Schilfgaarde}, R and J KEIZER",
year = "2003",
month = "11",
day = "1",
doi = "10.1002/jbm.a.10023",
language = "English",
volume = "67A",
pages = "679--683",
journal = "Journal of Biomedical Materials Research. Part A",
issn = "1549-3296",
publisher = "Wiley",
number = "2",

}

RIS

TY - JOUR

T1 - Effective removal of alginate-poly-L-lysine microcapsules from pancreatic islets by use of trypsin-EDTA

AU - de Groot, M

AU - Leuvenink, HGD

AU - Fekken, S

AU - Schuurs, TA

AU - van Schilfgaarde, R

AU - KEIZER, J

PY - 2003/11/1

Y1 - 2003/11/1

N2 - Although the transplantation of alginate-poly-L-lysine-alginate encapsulated islets of Langerhans usually is successful, graft survival is still limited. Molecular analysis by RT-PCR of the encapsulated islets may provide insight into the mechanisms that affect islets during graft failure. However, RT-PCR on encapsulated islets is not possible because the poly-L-lysine of the capsule interferes with both cDNA synthesis and PCR amplification. We applied a method that mechanically removes the microcapsules from the islets after a short trypsin-EDTA treatment (decapsulation), thereby enabling RT-PCR analysis. The results of this study show that the decapsulation procedure does not affect islet vitality and has only minor effects on islet function and morphology. The decapsulation does not affect GAPDH, beta-actin, Bcl-2, or Bax gene expression. This method is an improvement over the time-consuming manual dissection of microcapsules because it allows for the rapid and relatively harmless removal of capsules on a larger scale. Decapsulation offers the possibility of applying RT-PCR, as well as other methods, which cannot be performed on encapsulated islets. (C) 2003 Wiley Periodicals, Inc.

AB - Although the transplantation of alginate-poly-L-lysine-alginate encapsulated islets of Langerhans usually is successful, graft survival is still limited. Molecular analysis by RT-PCR of the encapsulated islets may provide insight into the mechanisms that affect islets during graft failure. However, RT-PCR on encapsulated islets is not possible because the poly-L-lysine of the capsule interferes with both cDNA synthesis and PCR amplification. We applied a method that mechanically removes the microcapsules from the islets after a short trypsin-EDTA treatment (decapsulation), thereby enabling RT-PCR analysis. The results of this study show that the decapsulation procedure does not affect islet vitality and has only minor effects on islet function and morphology. The decapsulation does not affect GAPDH, beta-actin, Bcl-2, or Bax gene expression. This method is an improvement over the time-consuming manual dissection of microcapsules because it allows for the rapid and relatively harmless removal of capsules on a larger scale. Decapsulation offers the possibility of applying RT-PCR, as well as other methods, which cannot be performed on encapsulated islets. (C) 2003 Wiley Periodicals, Inc.

KW - alginate-poly-L-lysine-alginate microcapsules

KW - islets of Langerhans

KW - trypsin

KW - EDTA

KW - RT-PCR

KW - PROPIDIUM IODIDE

KW - ACRIDINE-ORANGE

KW - BIOCOMPATIBILITY

KW - VIABILITY

KW - GRAFTS

KW - CELLS

U2 - 10.1002/jbm.a.10023

DO - 10.1002/jbm.a.10023

M3 - Article

VL - 67A

SP - 679

EP - 683

JO - Journal of Biomedical Materials Research. Part A

JF - Journal of Biomedical Materials Research. Part A

SN - 1549-3296

IS - 2

ER -

ID: 4143238