Publication

Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells

Petridis, X., Beems, B. P., Tomson, P., Scheven, B., Giepmans, B., Kuipers, J., van der Sluis, L. & Harmsen, M. C., 1-Aug-2019, In : Tissue Engineering. Part A. 25, 15-16, p. 1104-1115 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Petridis, X., Beems, B. P., Tomson, P., Scheven, B., Giepmans, B., Kuipers, J., ... Harmsen, M. C. (2019). Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells. Tissue Engineering. Part A, 25(15-16), 1104-1115. https://doi.org/10.1089/ten.TEA.2018.0192

Author

Petridis, Xenos ; Beems, Bas Pieter ; Tomson, Phillip ; Scheven, Ben ; Giepmans, Ben ; Kuipers, Jeroen ; van der Sluis, Luc ; Harmsen, Martin C. / Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells. In: Tissue Engineering. Part A. 2019 ; Vol. 25, No. 15-16. pp. 1104-1115.

Harvard

Petridis, X, Beems, BP, Tomson, P, Scheven, B, Giepmans, B, Kuipers, J, van der Sluis, L & Harmsen, MC 2019, 'Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells', Tissue Engineering. Part A, vol. 25, no. 15-16, pp. 1104-1115. https://doi.org/10.1089/ten.TEA.2018.0192

Standard

Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells. / Petridis, Xenos; Beems, Bas Pieter; Tomson, Phillip; Scheven, Ben; Giepmans, Ben; Kuipers, Jeroen; van der Sluis, Luc; Harmsen, Martin C.

In: Tissue Engineering. Part A, Vol. 25, No. 15-16, 01.08.2019, p. 1104-1115.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Petridis X, Beems BP, Tomson P, Scheven B, Giepmans B, Kuipers J et al. Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells. Tissue Engineering. Part A. 2019 Aug 1;25(15-16):1104-1115. https://doi.org/10.1089/ten.TEA.2018.0192


BibTeX

@article{12f78d91f3c8430aba9f0241291e7038,
title = "Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells",
abstract = "In teeth with an injured pulp, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs). dEMCs regulate the differentiation of resident mesenchymal stromal cells (MSCs), thereby affecting mineral deposition. In this study, we show that low-concentration solubilized dEMCs in osteogenic cultures of human umbilical cord mesenchymal stromal cells (UC-MSCs) and dental pulp stromal cells (DPSCs) enhanced mineral deposition, while adipose stromal cells (ASCs) were barely affected. Interestingly, UC-MSCs displayed significantly greater hydroxyapatite formation compared with DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMC-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large-sized mineral accretions on a poorly organized collagen network were the prominent feature of UC-MSC cultures, while mineral nodules interspersed throughout a collagen mesh were observed in the respective DPSC cultures. The ability of dEMCs to induce mineralization varies between different human MSC types in terms of total mineral formation and architecture. Mineral formation by UC-MSCs exposed to low-concentration dEMCs proved to be the most efficient and therefore could be considered a promising combination for mineralized tissue engineering. Impact Statement This research has been conducted with the aim to contribute to the development of treatment modalities for the reconstruction of lost/damaged mineralized tissues. Currently, determining the most appropriate stromal cell population and signaling cues stands at the core of developing effective treatments. We provide new insights into the effect of innate inductive cues found in human dentin matrix components, on the osteogenic differentiation of various human stromal cell types. The effects of dentin extracellular matrix components on umbilical cord mesenchymal stromal cells have not been investigated before. The findings of this study could underpin translational research based on the development of techniques for mineralized tissue engineering and will be of great interest for the readership of Tissue Engineering Part A.",
keywords = "dental pulp, dentin matrix, mesenchymal stromal cells, mineralization, tissue engineering, umbilical cord, ENGINEERING CURRENT STRATEGIES, PULP STEM-CELLS, BONE-MARROW, ADIPOSE-TISSUE, TGF-BETA, OSTEOGENIC DIFFERENTIATION, UMBILICAL-CORD, GROWTH-FACTOR, REGENERATION, ERK",
author = "Xenos Petridis and Beems, {Bas Pieter} and Phillip Tomson and Ben Scheven and Ben Giepmans and Jeroen Kuipers and {van der Sluis}, Luc and Harmsen, {Martin C}",
year = "2019",
month = "8",
day = "1",
doi = "10.1089/ten.TEA.2018.0192",
language = "English",
volume = "25",
pages = "1104--1115",
journal = "Tissue Engineering. Part A",
issn = "1937-3341",
publisher = "MARY ANN LIEBERT, INC",
number = "15-16",

}

RIS

TY - JOUR

T1 - Effect of Dentin Matrix Components on the Mineralization of Human Mesenchymal Stromal Cells

AU - Petridis, Xenos

AU - Beems, Bas Pieter

AU - Tomson, Phillip

AU - Scheven, Ben

AU - Giepmans, Ben

AU - Kuipers, Jeroen

AU - van der Sluis, Luc

AU - Harmsen, Martin C

PY - 2019/8/1

Y1 - 2019/8/1

N2 - In teeth with an injured pulp, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs). dEMCs regulate the differentiation of resident mesenchymal stromal cells (MSCs), thereby affecting mineral deposition. In this study, we show that low-concentration solubilized dEMCs in osteogenic cultures of human umbilical cord mesenchymal stromal cells (UC-MSCs) and dental pulp stromal cells (DPSCs) enhanced mineral deposition, while adipose stromal cells (ASCs) were barely affected. Interestingly, UC-MSCs displayed significantly greater hydroxyapatite formation compared with DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMC-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large-sized mineral accretions on a poorly organized collagen network were the prominent feature of UC-MSC cultures, while mineral nodules interspersed throughout a collagen mesh were observed in the respective DPSC cultures. The ability of dEMCs to induce mineralization varies between different human MSC types in terms of total mineral formation and architecture. Mineral formation by UC-MSCs exposed to low-concentration dEMCs proved to be the most efficient and therefore could be considered a promising combination for mineralized tissue engineering. Impact Statement This research has been conducted with the aim to contribute to the development of treatment modalities for the reconstruction of lost/damaged mineralized tissues. Currently, determining the most appropriate stromal cell population and signaling cues stands at the core of developing effective treatments. We provide new insights into the effect of innate inductive cues found in human dentin matrix components, on the osteogenic differentiation of various human stromal cell types. The effects of dentin extracellular matrix components on umbilical cord mesenchymal stromal cells have not been investigated before. The findings of this study could underpin translational research based on the development of techniques for mineralized tissue engineering and will be of great interest for the readership of Tissue Engineering Part A.

AB - In teeth with an injured pulp, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs). dEMCs regulate the differentiation of resident mesenchymal stromal cells (MSCs), thereby affecting mineral deposition. In this study, we show that low-concentration solubilized dEMCs in osteogenic cultures of human umbilical cord mesenchymal stromal cells (UC-MSCs) and dental pulp stromal cells (DPSCs) enhanced mineral deposition, while adipose stromal cells (ASCs) were barely affected. Interestingly, UC-MSCs displayed significantly greater hydroxyapatite formation compared with DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMC-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large-sized mineral accretions on a poorly organized collagen network were the prominent feature of UC-MSC cultures, while mineral nodules interspersed throughout a collagen mesh were observed in the respective DPSC cultures. The ability of dEMCs to induce mineralization varies between different human MSC types in terms of total mineral formation and architecture. Mineral formation by UC-MSCs exposed to low-concentration dEMCs proved to be the most efficient and therefore could be considered a promising combination for mineralized tissue engineering. Impact Statement This research has been conducted with the aim to contribute to the development of treatment modalities for the reconstruction of lost/damaged mineralized tissues. Currently, determining the most appropriate stromal cell population and signaling cues stands at the core of developing effective treatments. We provide new insights into the effect of innate inductive cues found in human dentin matrix components, on the osteogenic differentiation of various human stromal cell types. The effects of dentin extracellular matrix components on umbilical cord mesenchymal stromal cells have not been investigated before. The findings of this study could underpin translational research based on the development of techniques for mineralized tissue engineering and will be of great interest for the readership of Tissue Engineering Part A.

KW - dental pulp

KW - dentin matrix

KW - mesenchymal stromal cells

KW - mineralization

KW - tissue engineering

KW - umbilical cord

KW - ENGINEERING CURRENT STRATEGIES

KW - PULP STEM-CELLS

KW - BONE-MARROW

KW - ADIPOSE-TISSUE

KW - TGF-BETA

KW - OSTEOGENIC DIFFERENTIATION

KW - UMBILICAL-CORD

KW - GROWTH-FACTOR

KW - REGENERATION

KW - ERK

U2 - 10.1089/ten.TEA.2018.0192

DO - 10.1089/ten.TEA.2018.0192

M3 - Article

C2 - 30444193

VL - 25

SP - 1104

EP - 1115

JO - Tissue Engineering. Part A

JF - Tissue Engineering. Part A

SN - 1937-3341

IS - 15-16

ER -

ID: 67853315