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Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae

Kuravi, K., Nagotu, S., Krikken, A. M., Sjollema, K., Deckers, M., Erdmann, R., Veenhuis, M. & van der Klei, I. J., 1-Oct-2006, In : Journal of Cell Science. 119, 19, p. 3994 - 4001 8 p.

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Saccharomyces cerevisiae contains three dynamin-related-proteins, Vps1p, Dnm1p and Mgm1p. Previous data from glucose-grown VPS1 and DNM1 null mutants suggested that Vps1p, but not Dnm1p, plays a role in regulating peroxisome abundance. Here we show that deletion of DNM1 also results in reduction of peroxisome numbers. This was not observed in glucose-grown dnm1 cells, but was evident in cells grown in the presence of oleate. Similar observations were made in cells lacking Fis1p, a protein involved in Dnm1p function. Fluorescence microscopy of cells producing Dnm1-GFP or GFP-Fis1p demonstrated that both proteins had a dual localization on mitochondria and peroxisomes. Quantitative analysis revealed a greater reduction in peroxisome number in oleate-induced vps1 cells relative to dnm1 or fis1 cells. A significant fraction of oleate-induced vps1 cells still contained two or more peroxisomes. Conversely, almost all cells of a dnm1 vps1 double-deletion strain contained only one, enlarged peroxisome. This suggests that deletion of DNM1 reinforces the vps1 peroxisome phenotype. Time-lapse imaging indicated that during budding of dnm1 vps1 cells, the single peroxisome present in the mother cell formed long protrusions into the developing bud. This organelle divided at a very late stage of the budding process, possibly during cytokinesis.
Original languageEnglish
Pages (from-to)3994 - 4001
Number of pages8
JournalJournal of Cell Science
Volume119
Issue number19
Publication statusPublished - 1-Oct-2006

    Keywords

  • GTP Phosphohydrolases, GTP-Binding Proteins, Mitochondria, Mitochondrial Proteins, Models, Biological, Oleic Acid, Organelles, Organisms, Genetically Modified, Peroxisomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Tissue Distribution, Vesicular Transport Proteins, REGULATES MITOCHONDRIAL FISSION, ENDOPLASMIC-RETICULUM, MAMMALIAN-CELLS, BUDDING YEAST, GTPASE, MEMBRANE, BIOGENESIS, DIVISION, RECEPTOR, ACTIN

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