Dynamic temporal variations in bovine lactoferrin glycan structures

Valk-Weeber, R. L., Eshuis-de Ruiter, T., Dijkhuizen, L. & van Leeuwen, S. VAN., Feb-2020, In : Journal of Agricultural and Food Chemistry. 68, 2, p. 549-560 11 p., acs.jafc.9b06762.

Research output: Contribution to journalArticleAcademicpeer-review

It has been reported previously that glycosylation of bovine lactoferrin changes over time. A detailed structural overview of these changes over the whole course of lactation, including pre-dry period milk, is lacking. In this study, a high-throughput analysis method was applied to the glycoprofile of lactoferrin isolated from colostrum, mature and pre-dry period mature milk was analyzed over two subsequent lactation cycles for 8 cows from diverse genetic backgrounds. In addition, comparisons are made with commercial bovine lactoferrin samples. During the first 72 h, dynamic changes in lactoferrin glycosylation occurred. Shifts in the oligomannose distribution and the number of sialylated and fucosylated glycans was observed. In some cows, we observed (α2,3) linked sialic acid in the earliest colostrum samples. The glycoprofiles appeared stable from 1 month after delivery, as well as between cows. In addition, the glycosylation profiles of commercial lactoferrins isolated from pooled mature milk was stable over the seasons. Lactoferrin glycosylation in the pre-dry period resembles colostrum lactoferrin. The variations in lactoferrin glycosylation profiles, lactoferrin concentrations and other milk parameters, provide detailed information that potentially assists unraveling the functions and biosynthesis regulation of lactoferrin glycosylation.  .

Original languageEnglish
Article numberacs.jafc.9b06762
Pages (from-to)549-560
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Issue number2
Early online date12-Dec-2019
Publication statusPublished - Feb-2020


  • lactoferrin, colostrum, milk, N-glycosylation, (alpha2,3)-sialic acid

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