Dynamic combinatorial chemistry to identify binders of ThiT, an S-component of the energy-coupling factor transporter for thiamineMonjas, L., Swier, L. J. Y. M., Setyawati, I., Slotboom, D. J. & Hirsch, A. K. H., 20-Oct-2017, In : ChemMedChem. 12, 20, p. 1693-1696 4 p.
Research output: Contribution to journal › Article › Academic › peer-review
We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill-defined pocket, and one of the first reports for its application with an integral membrane protein as target.
|Number of pages||4|
|Publication status||Published - 20-Oct-2017|