DNA capture into the ClyA nanopore: Diffusion-limited versus reaction-limited processes

Nomidis, S. K., Hooyberghs, J., Maglia, G. & Carlon, E., 1-Aug-2018, In : Journal of Physics-Condensed Matter. 30, 30, 6 p., 304001.

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The capture and translocation of biomolecules through nanometer-scale pores are processes with a potential large number of applications, and hence they have been intensively studied in the recent years. The aim of this paper is to review existing models of the capture process by a nanopore, together with some recent experimental data of short single- and double-stranded DNA captured by Cytolysin~A (ClyA) nanopore. ClyA is a transmembrane protein of bacterial origin which has been recently engineered through site-specific mutations, to allow the translocation of double- and single-stranded DNA. A comparison between theoretical estimations and experiments suggests that for both cases the capture is a reaction-limited process. This is corroborated by the observed salt dependence of the capture rate, which we find to be in quantitative agreement with the theoretical predictions.

Original languageEnglish
Article number304001
Number of pages6
JournalJournal of Physics-Condensed Matter
Issue number30
Early online date12-Jun-2018
Publication statusPublished - 1-Aug-2018

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