Publication

Diversity of human small intestinal Streptococcus and Veillonella populations

van den Bogert, B., Erkus, O., Boekhorst, J., de Goffau, M., Smid, E. J., Zoetendal, E. G. & Kleerebezem, M., Aug-2013, In : FEMS Microbial Ecology. 85, 2, p. 376-388 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Bartholomeus van den Bogert
  • Oylum Erkus
  • Jos Boekhorst
  • Marcus de Goffau
  • Eddy J. Smid
  • Erwin G. Zoetendal
  • Michiel Kleerebezem

Molecular and cultivation approaches were employed to study the phylogenetic richness and temporal dynamics of Streptococcus and Veillonella populations in the small intestine. Microbial profiling of human small intestinal samples collected from four ileostomy subjects at four time points displayed abundant populations of Streptococcus spp. most affiliated with S.salivarius, S.thermophilus, and S.parasanguinis, as well as Veillonella spp. affiliated with V.atypica, V.parvula, V.dispar, and V.rogosae. Relative abundances varied per subject and time of sampling. Streptococcus and Veillonella isolates were cultured using selective media from ileostoma effluent samples collected at two time points from a single subject. The richness of the Streptococcus and Veillonella isolates was assessed at species and strain level by 16S rRNA gene sequencing and genetic fingerprinting, respectively. A total of 160 Streptococcus and 37 Veillonella isolates were obtained. Genetic fingerprinting differentiated seven Streptococcus lineages from ileostoma effluent, illustrating the strain richness within this ecosystem. The Veillonella isolates were represented by a single phylotype. Our study demonstrated that the small intestinal Streptococcus populations displayed considerable changes over time at the genetic lineage level because only representative strains of a single Streptococcus lineage could be cultivated from ileostoma effluent at both time points.

Original languageEnglish
Pages (from-to)376-388
Number of pages13
JournalFEMS Microbial Ecology
Volume85
Issue number2
Publication statusPublished - Aug-2013

    Keywords

  • Streptococcus spp, Veillonella spp, human small intestine, selective cultivation, genotyping, HUMAN GASTROINTESTINAL-TRACT, MULTIPLE SEQUENCE ALIGNMENT, DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, HIGH-THROUGHPUT, GUT MICROBIOME, MOLECULAR ANALYSIS, GENETIC DIVERSITY, IMMUNE-RESPONSES, ORAL-CAVITY

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