Dissecting specific and global transcriptional regulation of bacterial gene expression

Gerosa, L., Kochanowski, K., Heinemann, M. & Sauer, U., Apr-2013, In : Molecular Systems Biology. 9, 11 p., 658.

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Gene expression is regulated by specific transcriptional circuits but also by the global expression machinery as a function of growth. Simultaneous specific and global regulation thus constitutes an additional-but often neglected-layer of complexity in gene expression. Here, we develop an experimental-computational approach to dissect specific and global regulation in the bacterium Escherichia coli. By using fluorescent promoter reporters, we show that global regulation is growth rate dependent not only during steady state but also during dynamic changes in growth rate and can be quantified through two promoter-specific parameters. By applying our approach to arginine biosynthesis, we obtain a quantitative understanding of both specific and global regulation that allows accurate prediction of the temporal response to simultaneous perturbations in arginine availability and growth rate. We thereby uncover two principles of joint regulation: (i) specific regulation by repression dominates the transcriptional response during metabolic steady states, largely repressing the biosynthesis genes even when biosynthesis is required and (ii) global regulation sets the maximum promoter activity that is exploited during the transition between steady states.

Original languageEnglish
Article number658
Number of pages11
JournalMolecular Systems Biology
Publication statusPublished - Apr-2013


  • Arginine, Computer Simulation, Escherichia coli K12, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Genes, Reporter, Green Fluorescent Proteins, Kinetics, Models, Biological, Promoter Regions, Genetic, Transcription, Genetic

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