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Disruption of AKAP-PKA Interaction Induces Hypercontractility With Concomitant Increase in Proliferation Markers in Human Airway Smooth Muscle

Baarsma, H. A., Han, B., Poppinga, W. J., Driessen, S., Elzinga, C. R. S., Halayko, A. J., Meurs, H., Maarsingh, H. & Schmidt, M., 9-Apr-2020, In : Frontiers in Cell and Developmental Biology. 8, p. 165 13 p., 165.

Research output: Contribution to journalArticleAcademicpeer-review

With the ability to switch between proliferative and contractile phenotype, airway smooth muscle (ASM) cells can contribute to the progression of airway diseases such as asthma and chronic obstructive pulmonary disease (COPD), in which airway obstruction is associated with ASM hypertrophy and hypercontractility. A-kinase anchoring proteins (AKAPs) have emerged as important regulatory molecules in various tissues, including ASM cells. AKAPs can anchor the regulatory subunits of protein kinase A (PKA), and guide cellular localization via various targeting domains. Here we investigated whether disruption of the AKAP-PKA interaction, by the cell permeable peptide stearated (st)-Ht31, alters human ASM proliferation and contractility. Treatment of human ASM with st-Ht31 enhanced the expression of protein markers associated with cell proliferation in both cultured cells and intact tissue, although this was not accompanied by an increase in cell viability or cell-cycle progression, suggesting that disruption of AKAP-PKA interaction on its own is not sufficient to drive ASM cell proliferation. Strikingly, st-Ht31 enhanced contractile force generation in human ASM tissue with concomitant upregulation of the contractile protein alpha-sm-actin. This upregulation of alpha-sm-actin was independent of mRNA stability, transcription or translation, but was dependent on proteasome function, as the proteasome inhibitor MG-132 prevented the st-Ht31 effect. Collectively, the AKAP-PKA interaction appears to regulate markers of the multi-functional capabilities of ASM, and this alter the physiological function, such as contractility, suggesting potential to contribute to the pathophysiology of airway diseases.

Original languageEnglish
Article number165
Pages (from-to)165
Number of pages13
JournalFrontiers in Cell and Developmental Biology
Volume8
Publication statusPublished - 9-Apr-2020

    Keywords

  • airway smooth muscle, A-kinase anchoring proteins (AKAP), protein kinase A (PKA), asthma, chronic obstructive pulmonary disease (COPD), KINASE-ANCHORING PROTEINS, CYCLIN D1 EXPRESSION, DNA-SYNTHESIS, CELL-CYCLE, SIGNALING COMPLEXES, REGULATORY SUBUNIT, CAMP, PHENOTYPE, GROWTH, ACTIVATION

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