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Differential role for very late antigen-5 in mobilization and homing of hematopoietic stem cells

Wierenga, P. K., Weersing, E., Dontje, B., de Haan, G. & van Os, R., Dec-2006, In : Bone marrow transplantation. 38, 12, p. 789-797 9 p.

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  • Wierenga et al BMT 2006

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DOI

The role of very late antigen-5 (VLA-5) in homing and mobilization of hematopoietic stem cells from normal bone marrow (NBM) and bone marrow (MBM) and peripheral blood (MPB) from mobilized mice was investigated. We found a decreased number of VLA-5-expressing cells in the lineage-negative fraction of MPB. However, virtually all stem/progenitor cells were present in the VLA- 5(+) fraction and hence mobilization of hematopoietic stem cell subsets does not coincide with a downregulation of VLA-5. Stem/progenitor cells from MPB and MBM demonstrated enhanced stromal-derived factor-alpha-induced migration. This enhanced migration correlates with an improved hematopoietic reconstitution potential, with the migrated MPB cells showing the fastest reconstitution. Interestingly, homing of MPB, MBM and NBM stem/progenitor cells in bone marrow and spleen did not differ and is therefore not responsible for the differences in hematopoietic reconstitution. The observed increase in VLA- 5(+) cells in the recipients after transplantation can most probably be attributed to selective homing of VLA- 5(+) cells instead of an upregulation of VLA-5. Treatment with an antibody to VLA-5 partially inhibited bone marrow homing of progenitor cells, whereas homing in the spleen was hardly affected. These data indicate a differential role for VLA- 5 in the movement of stem cells from and toward bone marrow.

Original languageEnglish
Pages (from-to)789-797
Number of pages9
JournalBone marrow transplantation
Volume38
Issue number12
Publication statusPublished - Dec-2006

    Keywords

  • VLA-5, mobilization, homing, BLOOD PROGENITOR CELLS, ADHESION MOLECULE EXPRESSION, COLONY-STIMULATING FACTOR, ACUTE MYELOID-LEUKEMIA, BONE-MARROW-CELLS, PERIPHERAL-BLOOD, NOD/SCID MICE, INTEGRIN EXPRESSION, CD34(+) CELLS, REPOPULATING CELLS

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