Publication

Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions

Price, C. E., Otto, A., Fusetti, F., Becher, D., Hecker, M. & Driessen, A. J. M., Sep-2010, In : Proteomics. 10, 18, p. 3235 - 3247 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Price, C. E., Otto, A., Fusetti, F., Becher, D., Hecker, M., & Driessen, A. J. M. (2010). Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. Proteomics, 10(18), 3235 - 3247. https://doi.org/10.1002/pmic.201000284

Author

Price, Claire E. ; Otto, Andreas ; Fusetti, Fabrizia ; Becher, Doerte ; Hecker, Michael ; Driessen, Arnold J. M. / Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. In: Proteomics. 2010 ; Vol. 10, No. 18. pp. 3235 - 3247.

Harvard

Price, CE, Otto, A, Fusetti, F, Becher, D, Hecker, M & Driessen, AJM 2010, 'Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions', Proteomics, vol. 10, no. 18, pp. 3235 - 3247. https://doi.org/10.1002/pmic.201000284

Standard

Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. / Price, Claire E.; Otto, Andreas; Fusetti, Fabrizia; Becher, Doerte; Hecker, Michael; Driessen, Arnold J. M.

In: Proteomics, Vol. 10, No. 18, 09.2010, p. 3235 - 3247.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Price CE, Otto A, Fusetti F, Becher D, Hecker M, Driessen AJM. Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. Proteomics. 2010 Sep;10(18):3235 - 3247. https://doi.org/10.1002/pmic.201000284


BibTeX

@article{b0339ec3b6bf4bb48eb95c97ef310479,
title = "Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions",
abstract = "YidC of Escherichia coli belongs to the evolutionarily conserved Oxa1/Alb3/YidC family. Members of the family have all been implicated in membrane protein biogenesis of respiratory and energy transducing proteins. The number of proteins identified thus far to require YidC for their membrane biogenesis remains limited and the identification of new substrates may allow the elucidation of properties that define the YidC specificity. To this end we investigated changes in the membrane proteome of E. coli upon YidC depletion using metabolic labeling of proteins with (15)N/(14)N combined with a MS-centered proteomics approach and compared the effects of YidC depletion under aerobic and anaerobic growth conditions. We found that YidC depletion resulted in protein aggregation/misfolding in the cytoplasm as well as in the inner membrane of E. coli. A dramatic increase was observed in the chaperone-mediated stress response upon YidC depletion and this response was limited to aerobically grown cells. A number of transporter proteins were identified as possible candidates for the YidC-dependent insertion and/or folding pathway. These included the small metal ion transporter CorA, numerous ABC transporters, as well as the MFS transporters KgtP and ProP, providing a new subset of proteins potentially requiring YidC for membrane biogenesis.",
keywords = "Membrane protein, Microbiology, Phage shock protein A, YidC, SMALL HEAT-SHOCK, INNER MEMBRANE, SUBUNIT-C, MECHANOSENSITIVE CHANNEL, PREPROTEIN TRANSLOCASE, QUANTITATIVE-ANALYSIS, IN-VIVO, PROTEINS, INSERTION, COMPLEX",
author = "Price, {Claire E.} and Andreas Otto and Fabrizia Fusetti and Doerte Becher and Michael Hecker and Driessen, {Arnold J. M.}",
year = "2010",
month = sep,
doi = "10.1002/pmic.201000284",
language = "English",
volume = "10",
pages = "3235 -- 3247",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley",
number = "18",

}

RIS

TY - JOUR

T1 - Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions

AU - Price, Claire E.

AU - Otto, Andreas

AU - Fusetti, Fabrizia

AU - Becher, Doerte

AU - Hecker, Michael

AU - Driessen, Arnold J. M.

PY - 2010/9

Y1 - 2010/9

N2 - YidC of Escherichia coli belongs to the evolutionarily conserved Oxa1/Alb3/YidC family. Members of the family have all been implicated in membrane protein biogenesis of respiratory and energy transducing proteins. The number of proteins identified thus far to require YidC for their membrane biogenesis remains limited and the identification of new substrates may allow the elucidation of properties that define the YidC specificity. To this end we investigated changes in the membrane proteome of E. coli upon YidC depletion using metabolic labeling of proteins with (15)N/(14)N combined with a MS-centered proteomics approach and compared the effects of YidC depletion under aerobic and anaerobic growth conditions. We found that YidC depletion resulted in protein aggregation/misfolding in the cytoplasm as well as in the inner membrane of E. coli. A dramatic increase was observed in the chaperone-mediated stress response upon YidC depletion and this response was limited to aerobically grown cells. A number of transporter proteins were identified as possible candidates for the YidC-dependent insertion and/or folding pathway. These included the small metal ion transporter CorA, numerous ABC transporters, as well as the MFS transporters KgtP and ProP, providing a new subset of proteins potentially requiring YidC for membrane biogenesis.

AB - YidC of Escherichia coli belongs to the evolutionarily conserved Oxa1/Alb3/YidC family. Members of the family have all been implicated in membrane protein biogenesis of respiratory and energy transducing proteins. The number of proteins identified thus far to require YidC for their membrane biogenesis remains limited and the identification of new substrates may allow the elucidation of properties that define the YidC specificity. To this end we investigated changes in the membrane proteome of E. coli upon YidC depletion using metabolic labeling of proteins with (15)N/(14)N combined with a MS-centered proteomics approach and compared the effects of YidC depletion under aerobic and anaerobic growth conditions. We found that YidC depletion resulted in protein aggregation/misfolding in the cytoplasm as well as in the inner membrane of E. coli. A dramatic increase was observed in the chaperone-mediated stress response upon YidC depletion and this response was limited to aerobically grown cells. A number of transporter proteins were identified as possible candidates for the YidC-dependent insertion and/or folding pathway. These included the small metal ion transporter CorA, numerous ABC transporters, as well as the MFS transporters KgtP and ProP, providing a new subset of proteins potentially requiring YidC for membrane biogenesis.

KW - Membrane protein

KW - Microbiology

KW - Phage shock protein A

KW - YidC

KW - SMALL HEAT-SHOCK

KW - INNER MEMBRANE

KW - SUBUNIT-C

KW - MECHANOSENSITIVE CHANNEL

KW - PREPROTEIN TRANSLOCASE

KW - QUANTITATIVE-ANALYSIS

KW - IN-VIVO

KW - PROTEINS

KW - INSERTION

KW - COMPLEX

U2 - 10.1002/pmic.201000284

DO - 10.1002/pmic.201000284

M3 - Article

VL - 10

SP - 3235

EP - 3247

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 18

ER -

ID: 1933792