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Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions

Price, C. E., Otto, A., Fusetti, F., Becher, D., Hecker, M. & Driessen, A. J. M., Sep-2010, In : Proteomics. 10, 18, p. 3235 - 3247 13 p.

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  • Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions

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DOI

YidC of Escherichia coli belongs to the evolutionarily conserved Oxa1/Alb3/YidC family. Members of the family have all been implicated in membrane protein biogenesis of respiratory and energy transducing proteins. The number of proteins identified thus far to require YidC for their membrane biogenesis remains limited and the identification of new substrates may allow the elucidation of properties that define the YidC specificity. To this end we investigated changes in the membrane proteome of E. coli upon YidC depletion using metabolic labeling of proteins with (15)N/(14)N combined with a MS-centered proteomics approach and compared the effects of YidC depletion under aerobic and anaerobic growth conditions. We found that YidC depletion resulted in protein aggregation/misfolding in the cytoplasm as well as in the inner membrane of E. coli. A dramatic increase was observed in the chaperone-mediated stress response upon YidC depletion and this response was limited to aerobically grown cells. A number of transporter proteins were identified as possible candidates for the YidC-dependent insertion and/or folding pathway. These included the small metal ion transporter CorA, numerous ABC transporters, as well as the MFS transporters KgtP and ProP, providing a new subset of proteins potentially requiring YidC for membrane biogenesis.

Original languageEnglish
Pages (from-to)3235 - 3247
Number of pages13
JournalProteomics
Volume10
Issue number18
Publication statusPublished - Sep-2010

    Keywords

  • Membrane protein, Microbiology, Phage shock protein A, YidC, SMALL HEAT-SHOCK, INNER MEMBRANE, SUBUNIT-C, MECHANOSENSITIVE CHANNEL, PREPROTEIN TRANSLOCASE, QUANTITATIVE-ANALYSIS, IN-VIVO, PROTEINS, INSERTION, COMPLEX

ID: 1933792