Dietary Isomalto/Malto-Polysaccharides Increase Fecal Bulk and Microbial Fermentation in Mice

Mistry, R. H., Borewicz, K., Gu, F., Verkade, H. J., Schols, H. A., Smidt, H. & Tietge, U. J. F., 13-May-2020, In : Molecular Nutrition & Food Research. p. e2000251 10 p., 2000251.

Research output: Contribution to journalArticleAcademicpeer-review

Scope The prevalence of metabolic-syndrome-related disease has strongly increased. Nutritional intervention strategies appear attractive, particularly with novel prebiotics. Isomalto/malto-polysaccharides (IMMPs) represent promising novel prebiotics that promote proliferation of beneficial bacteria in vitro. The present study investigates for the first time the in vivo effects of IMMP in mice.

Methods and results C57BL/6 wild-type mice received control or IMMP-containing (10%, w/w) diets for 3 weeks. IMMP leads to significantly more fecal bulk (+26%, p <0.05), higher plasma non-esterified fatty acids (colorimetric assay, +10%, p <0.05), and lower fecal dihydrocholesterol excretion (mass spectrometry, -50%, p <0.05). Plasma and hepatic lipid levels (colorimetric assays following lipid extraction) are not influenced by dietary IMMP, as are other parameters of sterol metabolism, including bile acids (gas chromatography/mass spectrometry). IMMP is mainly fermented in the cecum and large intestine (high-performance anion exchange chromatography). Next-generation sequencing demonstrates higher relative abundance of Bacteroides and butyrate producers (Lachnospiraceae, Roseburia Odoribacter) in the IMMP group.

Conclusion The combined results demonstrate that IMMP administration to mice increases fecal bulk and induces potentially beneficial changes in the intestinal microbiota. Further studies are required in disease models to substantiate potential health benefits.

Original languageEnglish
Article number2000251
Pages (from-to)e2000251
Number of pages10
JournalMolecular Nutrition & Food Research
Early online date25-Apr-2020
Publication statusPublished - 13-May-2020


  • bile acids, cholesterol, IMMP, microbiota, polysaccharides, prebiotics, short-chain fatty acids, CHAIN FATTY-ACIDS, GERM-FREE MICE, FIBER INTAKE, CHOLESTEROL, GUT, PROPIONATE, METABOLISM

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