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Development of a Novel Orthogonal Double Gradient for High-Throughput Screening of Mesenchymal Stem Cells-Materials Interaction

Zhou, Q., Ge, L., Guimaraes, C. F., Kuhn, P. T., Yang, L. & van Rijn, P., 21-Sep-2018, In : Advanced Materials Interfaces. 5, 18, 8 p., 1800504.

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  • Development of a Novel Orthogonal Double Gradient for High‐Throughput Screening of Mesenchymal Stem Cells–Materials Interaction

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DOI

Surface gradients provide a powerful platform to accelerate multiscale design by efficiently studying of material-cell interactions to ultimately enhance function of synthetic clinical biomaterials. Herein, a novel orthogonal double gradient is reported in which surface stiffness and wettability vary independently and continuously in perpendicular directions, providing unique combinations of stiffness and wettability over a broad range (stiffness: 6-89 MPa; water contact angle: 29 degrees-90 degrees). It is found that mesenchymal stem cell behavior is nonlinearly regulated by surface stiffness and wettability. These combined stiffness and wettability properties of a material significantly affect stem cell adhesion, spreading, nucleus size, and vinculin expression. This high-throughput screening system enables elucidation of the relationships between biointerface properties and biological behavior, and thereby serves a potential tool for accelerating the development of high-performance biomaterials.

Original languageEnglish
Article number1800504
Number of pages8
JournalAdvanced Materials Interfaces
Volume5
Issue number18
Publication statusPublished - 21-Sep-2018

    Keywords

  • high-throughput screening, orthogonal double gradient, stem cell, stiffness, wettability, CHEMICAL GRADIENTS, CONTACT GUIDANCE, POLYMER SURFACES, ADHESION, DIFFERENTIATION, SUBSTRATE, STIFFNESS, MATRIX, MICROENVIRONMENTS, BIOMATERIALS

ID: 65980491