Development of a Novel Orthogonal Double Gradient for High-Throughput Screening of Mesenchymal Stem Cells-Materials InteractionZhou, Q., Ge, L., Guimaraes, C. F., Kuhn, P. T., Yang, L. & van Rijn, P., 21-Sep-2018, In : Advanced Materials Interfaces. 5, 18, 8 p., 1800504.
Research output: Contribution to journal › Article › Academic › peer-review
Surface gradients provide a powerful platform to accelerate multiscale design by efficiently studying of material-cell interactions to ultimately enhance function of synthetic clinical biomaterials. Herein, a novel orthogonal double gradient is reported in which surface stiffness and wettability vary independently and continuously in perpendicular directions, providing unique combinations of stiffness and wettability over a broad range (stiffness: 6-89 MPa; water contact angle: 29 degrees-90 degrees). It is found that mesenchymal stem cell behavior is nonlinearly regulated by surface stiffness and wettability. These combined stiffness and wettability properties of a material significantly affect stem cell adhesion, spreading, nucleus size, and vinculin expression. This high-throughput screening system enables elucidation of the relationships between biointerface properties and biological behavior, and thereby serves a potential tool for accelerating the development of high-performance biomaterials.
|Number of pages||8|
|Journal||Advanced Materials Interfaces|
|Publication status||Published - 21-Sep-2018|
- high-throughput screening, orthogonal double gradient, stem cell, stiffness, wettability, CHEMICAL GRADIENTS, CONTACT GUIDANCE, POLYMER SURFACES, ADHESION, DIFFERENTIATION, SUBSTRATE, STIFFNESS, MATRIX, MICROENVIRONMENTS, BIOMATERIALS