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Detection of Two Isomeric Binding Configurations in a Protein-Aptamer Complex with a Biological Nanopore

Van Meervelt, V., Soskine, M. & Maglia, G., Dec-2014, In : Acs Nano. 8, 12, p. 12826-12835 10 p.

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  • Detection of Two Isomeric Binding Configurations in a Protein-Aptamer Complex with a Biological Nanopore

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DOI

ProteinDNA interactions play critical roles in biological systems, and they often involve complex mechanisms and dynamics that are not easily measured by ensemble experiments. Recently, we showed that folded proteins can be internalized inside ClyA nanopores and studied by ionic current recordings at the single-molecule level. Here, we use ClyA nanopores to sample the interaction between the G-quadruplex fold of the thrombin binding aptamer (TBA) and human thrombin (HT). Surprisingly, the internalization of the HT:TBA complex inside the nanopore induced two types of current blockades with distinguished residual current and lifetime. Using single nucleobase substitutions to TBA we showed that these two types of blockades originate from TBA binding to thrombin with two isomeric orientations. Voltage dependencies and the use of ClyA nanopores with two different diameters allowed assessing the effect of the applied potential and confinement and revealed that the two binding configurations of TBA to HT display different lifetimes. These results show that the ClyA nanopores can be used to probe conformational heterogeneity in protein:DNA interactions.

Original languageEnglish
Pages (from-to)12826-12835
Number of pages10
JournalAcs Nano
Volume8
Issue number12
Publication statusPublished - Dec-2014

    Keywords

  • single-molecule, nanopore, protein:DNA interactions, thrombin, aptamer, SOLID-STATE NANOPORES, AFFINITY CAPILLARY-ELECTROPHORESIS, G-QUADRUPLEX STRUCTURES, SINGLE-MOLECULE, ALPHA-HEMOLYSIN, DNA INTERFACES, THROMBIN, TRANSLOCATION, TRANSCRIPTION, PORE

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