Detection of Toxic Phytoplankton Species by Immunochemical Particle Analysis Based on Flow CytometryVrieling, E. G., Gieskes, W. W. C., Zeijl, W. J. M. V. & Veenhuis, M., 1994, In : Netherlands journal of aquatic ecology. 28, 3, 6 p.
Research output: Contribution to journal › Article › Academic
Particulate suspended matter in oceanic, coastal, and estuarine regions can be specifically marked immunochemically with a fluorescent probe using antisera recognizing antigens present on their surface. Of the particulate matter, phytoplankton is a major component. Toxic species that may form harmful blooms can be a direct threat to aquaculturing, tourism, sea-life and man. In order to detect such species in natural fixed phytoplankton populations, immunochemical tagging has been combined with flow cytometric evaluation. Microalgal cells can be labeled with a fluorescent probe (fluorescein isothiocyanate, FITC, is recommended). Labeled cells are counted using a flow cytometer. This method has proved to be applicable in a monitoring programme in the North Sea.
|Number of pages||6|
|Journal||Netherlands journal of aquatic ecology|
|Publication status||Published - 1994|
- toxic phytoplankton, immunochemical labeling, flow cytometry, dinoflagellates
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