Publication

Detection of infliximab levels and anti-infliximab antibodies: a comparison of three different assays

Casteele, N. V., Buurman, D. J., Sturkenboom, M. G. G., Kleibeuker, J. H., Vermeire, S., Rispens, T., van der Kleij, D., Gils, A. & Dijkstra, G., Oct-2012, In : Alimentary Pharmacology & Therapeutics. 36, 8, p. 765-771 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Background Formation of antibodies to infliximab (ATI) inversely correlates with functional drug levels and clinical outcome. Comparison of drug levels and anti-drug antibody monitoring is hampered by lack of standardisation. Aim To determine the correlation between three different assays for measuring infliximab and ATI. Methods Serum samples and spiked controls (total 62) were evaluated in a blinded way in infliximab and ATI assays developed by Sanquin Amsterdam, Netherlands (A), Laboratory for Pharmaceutical Biology, KU Leuven, Belgium (B) and a commercially available kit from Biomedical Diagnostics (BMD), Paris, France (C) performed by the University Medical Center Groningen (UMCG), Netherlands. Results All infliximab assays showed a linear quantitative correlation (Pearson r = 0.91 for A vs. B, 0.83 for A vs. C and 0.73 for B vs. C). Assay C detected infliximab in 11 samples (18%) not detected by A and B, including samples containing only ATI. All ATI assays showed a good linear correlation (Pearson r = 0.95 for A vs. B, 0.99 for A vs. C and 0.97 for B vs. C). Assay A detected ATI in five samples with low ATI that were not detected by assays B and C. Assay B did not detect ATI in three patient samples with low ATI according to assays A and C. Conclusions There is a good correlation of infliximab and antibodies to infliximab measurements between these assays. Nevertheless, the Biomedical Diagnostics kit detected false positive infliximab levels in 18% of the samples.

Original languageEnglish
Pages (from-to)765-771
Number of pages7
JournalAlimentary Pharmacology & Therapeutics
Volume36
Issue number8
Publication statusPublished - Oct-2012

    Keywords

  • CROHNS-DISEASE, MONOCLONAL-ANTIBODY, ULCERATIVE-COLITIS, RHEUMATOID-ARTHRITIS, CLINICAL-RESPONSE, ADALIMUMAB, IMMUNOGENICITY, INDUCTION, TRIAL, TERM

ID: 5669609