Publication

Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study

Eijsink, J. J. H., Yang, N., Lendvai, A., Klip, H. G., Volders, H. H., Buikema, H. J., van Hemel, B. M., Voll, M., Bennink, H. J. T. C., Schuuring, E., Wisman, G. B. A. & van der Zee, A. G. J., Feb-2011, In : Gynecologic Oncology. 120, 2, p. 280-283 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Eijsink, J. J. H., Yang, N., Lendvai, A., Klip, H. G., Volders, H. H., Buikema, H. J., van Hemel, B. M., Voll, M., Bennink, H. J. T. C., Schuuring, E., Wisman, G. B. A., & van der Zee, A. G. J. (2011). Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study. Gynecologic Oncology, 120(2), 280-283. https://doi.org/10.1016/j.ygyno.2010.10.029

Author

Eijsink, J. J. H. ; Yang, N. ; Lendvai, A. ; Klip, H. G. ; Volders, H. H. ; Buikema, H. J. ; van Hemel, B. M. ; Voll, M. ; Bennink, H. J. T. Coelingh ; Schuuring, E. ; Wisman, G. B. A. ; van der Zee, A. G. J. / Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study. In: Gynecologic Oncology. 2011 ; Vol. 120, No. 2. pp. 280-283.

Harvard

Eijsink, JJH, Yang, N, Lendvai, A, Klip, HG, Volders, HH, Buikema, HJ, van Hemel, BM, Voll, M, Bennink, HJTC, Schuuring, E, Wisman, GBA & van der Zee, AGJ 2011, 'Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study', Gynecologic Oncology, vol. 120, no. 2, pp. 280-283. https://doi.org/10.1016/j.ygyno.2010.10.029

Standard

Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study. / Eijsink, J. J. H.; Yang, N.; Lendvai, A.; Klip, H. G.; Volders, H. H.; Buikema, H. J.; van Hemel, B. M.; Voll, M.; Bennink, H. J. T. Coelingh; Schuuring, E.; Wisman, G. B. A.; van der Zee, A. G. J.

In: Gynecologic Oncology, Vol. 120, No. 2, 02.2011, p. 280-283.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Eijsink JJH, Yang N, Lendvai A, Klip HG, Volders HH, Buikema HJ et al. Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study. Gynecologic Oncology. 2011 Feb;120(2):280-283. https://doi.org/10.1016/j.ygyno.2010.10.029


BibTeX

@article{d2e10c5871d24a1c87d2de87805f35e2,
title = "Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study",
abstract = "Objective. To explore the feasibility of DNA methylation analysis for the detection of cervical neoplasia in self-obtained cervico-vaginal lavages.Methods. Lavages collected by a self-sampling device and paired cervical scrapings were obtained from 20 cervical cancer patients and 23 patients referred with an abnormal cervical smear (15 with high-grade cervical intraepithelial neoplasia (CIN2+) and 8 without CIN). All lavages and scrapings were analyzed by liquid based cytology (LBC), Hybrid Capture II (HC-II) for hr-HPV DNA detection and by DNA methylation analysis (JAM3, TERT, EPB41L3 and C13ORF18). Concordance between lavages and scrapings was measured by Cohen's Kappa (k).Results. In lavages and scrapings from cervical cancer patients (n =20), methylation analysis was positive in 19 (95%) and 19 (95%), HC-II in 16 (80%) and 15 (75%) and LBC in 15 (75%) and 19 (95%), respectively. In lavages and scrapings from CIN2+ patients (n = 15), methylation analysis was positive in 10 (67%) and 12 (80%), HC-II in 15 (100%) and 15 (100%) and LBC in 11 (73%) and 12 (80%), respectively. Concordance between cervical scrapings and lavages (n = 43) was for LBC k = 0.522 (pConclusions. DNA methylation analysis in cervico-vaginal lavages obtained by a self-sampling device is feasible and its diagnostic performance appears to be at least comparable to the detection of cervical neoplasia by cytomorphology and hr-HPV. Our pilot study suggests that detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages warrants exploration of its use in large prospective studies. (C) 2010 Elsevier Inc. All rights reserved.",
keywords = "Cervical, Neoplasia, Self-sampling, Lavage, Cervical cancer, Methylation markers, HUMAN-PAPILLOMAVIRUS DNA, INTRAEPITHELIAL NEOPLASIA, CANCER, NETHERLANDS, CYTOLOGY, MARKERS, WOMEN",
author = "Eijsink, {J. J. H.} and N. Yang and A. Lendvai and Klip, {H. G.} and Volders, {H. H.} and Buikema, {H. J.} and {van Hemel}, {B. M.} and M. Voll and Bennink, {H. J. T. Coelingh} and E. Schuuring and Wisman, {G. B. A.} and {van der Zee}, {A. G. J.}",
year = "2011",
month = feb,
doi = "10.1016/j.ygyno.2010.10.029",
language = "English",
volume = "120",
pages = "280--283",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "2",

}

RIS

TY - JOUR

T1 - Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study

AU - Eijsink, J. J. H.

AU - Yang, N.

AU - Lendvai, A.

AU - Klip, H. G.

AU - Volders, H. H.

AU - Buikema, H. J.

AU - van Hemel, B. M.

AU - Voll, M.

AU - Bennink, H. J. T. Coelingh

AU - Schuuring, E.

AU - Wisman, G. B. A.

AU - van der Zee, A. G. J.

PY - 2011/2

Y1 - 2011/2

N2 - Objective. To explore the feasibility of DNA methylation analysis for the detection of cervical neoplasia in self-obtained cervico-vaginal lavages.Methods. Lavages collected by a self-sampling device and paired cervical scrapings were obtained from 20 cervical cancer patients and 23 patients referred with an abnormal cervical smear (15 with high-grade cervical intraepithelial neoplasia (CIN2+) and 8 without CIN). All lavages and scrapings were analyzed by liquid based cytology (LBC), Hybrid Capture II (HC-II) for hr-HPV DNA detection and by DNA methylation analysis (JAM3, TERT, EPB41L3 and C13ORF18). Concordance between lavages and scrapings was measured by Cohen's Kappa (k).Results. In lavages and scrapings from cervical cancer patients (n =20), methylation analysis was positive in 19 (95%) and 19 (95%), HC-II in 16 (80%) and 15 (75%) and LBC in 15 (75%) and 19 (95%), respectively. In lavages and scrapings from CIN2+ patients (n = 15), methylation analysis was positive in 10 (67%) and 12 (80%), HC-II in 15 (100%) and 15 (100%) and LBC in 11 (73%) and 12 (80%), respectively. Concordance between cervical scrapings and lavages (n = 43) was for LBC k = 0.522 (pConclusions. DNA methylation analysis in cervico-vaginal lavages obtained by a self-sampling device is feasible and its diagnostic performance appears to be at least comparable to the detection of cervical neoplasia by cytomorphology and hr-HPV. Our pilot study suggests that detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages warrants exploration of its use in large prospective studies. (C) 2010 Elsevier Inc. All rights reserved.

AB - Objective. To explore the feasibility of DNA methylation analysis for the detection of cervical neoplasia in self-obtained cervico-vaginal lavages.Methods. Lavages collected by a self-sampling device and paired cervical scrapings were obtained from 20 cervical cancer patients and 23 patients referred with an abnormal cervical smear (15 with high-grade cervical intraepithelial neoplasia (CIN2+) and 8 without CIN). All lavages and scrapings were analyzed by liquid based cytology (LBC), Hybrid Capture II (HC-II) for hr-HPV DNA detection and by DNA methylation analysis (JAM3, TERT, EPB41L3 and C13ORF18). Concordance between lavages and scrapings was measured by Cohen's Kappa (k).Results. In lavages and scrapings from cervical cancer patients (n =20), methylation analysis was positive in 19 (95%) and 19 (95%), HC-II in 16 (80%) and 15 (75%) and LBC in 15 (75%) and 19 (95%), respectively. In lavages and scrapings from CIN2+ patients (n = 15), methylation analysis was positive in 10 (67%) and 12 (80%), HC-II in 15 (100%) and 15 (100%) and LBC in 11 (73%) and 12 (80%), respectively. Concordance between cervical scrapings and lavages (n = 43) was for LBC k = 0.522 (pConclusions. DNA methylation analysis in cervico-vaginal lavages obtained by a self-sampling device is feasible and its diagnostic performance appears to be at least comparable to the detection of cervical neoplasia by cytomorphology and hr-HPV. Our pilot study suggests that detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages warrants exploration of its use in large prospective studies. (C) 2010 Elsevier Inc. All rights reserved.

KW - Cervical

KW - Neoplasia

KW - Self-sampling

KW - Lavage

KW - Cervical cancer

KW - Methylation markers

KW - HUMAN-PAPILLOMAVIRUS DNA

KW - INTRAEPITHELIAL NEOPLASIA

KW - CANCER

KW - NETHERLANDS

KW - CYTOLOGY

KW - MARKERS

KW - WOMEN

U2 - 10.1016/j.ygyno.2010.10.029

DO - 10.1016/j.ygyno.2010.10.029

M3 - Article

VL - 120

SP - 280

EP - 283

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 2

ER -

ID: 5260383